Antiviral Effects of Pomegranate Peel Extracts on Human Norovirus in Food Models and Simulated Gastrointestinal Fluids.
Antiviral effect
Food model
Human norovirus
Pomegranate peel extract
Journal
Plant foods for human nutrition (Dordrecht, Netherlands)
ISSN: 1573-9104
Titre abrégé: Plant Foods Hum Nutr
Pays: Netherlands
ID NLM: 8803554
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
accepted:
24
03
2021
pubmed:
8
4
2021
medline:
2
7
2021
entrez:
7
4
2021
Statut:
ppublish
Résumé
Human noroviruses (HuNoV) are the dominant cause of viral gastroenteritis in all age groups worldwide. In this study, we investigated the effects of pomegranate peel extract (PPE) on the reduction of HuNoV in different food models, on surfaces of fresh produce (green onion and cherry tomato), in low-fat milk, and simulated gastrointestinal fluids. The antiviral efficacy of PPE against HuNoV was evaluated by quantifying the number of residual virus genomes using a quantitative reverse transcription PCR (qRT-PCR) assay. Pomegranate peel, considered as a waste product of industrial processing, is known for beneficial health effects and broad antimicrobial activity due to the high content of phenolic compounds and tannins. PPE showed significant antiviral properties against HuNoV both in phosphate-buffered saline (PBS) and simulated gastric fluid. The reduction of HuNoV by pomegranate juice was lower than with PPE, which could be attributed to the lower content of antimicrobial compounds. A pretreatment of cherry tomato and green onion surfaces with PPE significantly reduced the amount of HuNoV particles that adhered to those surfaces during subsequent virus suspension treatment. A detrimental effect of PPE on HuNoV structure was confirmed by transmission electron microscopy. Our results indicate that PPE is a natural antiviral agent effective against food-borne noroviruses.
Identifiants
pubmed: 33825092
doi: 10.1007/s11130-021-00895-7
pii: 10.1007/s11130-021-00895-7
pmc: PMC8024177
doi:
Substances chimiques
Antiviral Agents
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
203-209Références
Rowe G, Bolger F (2016) Final report on ‘the identification of food safety priorities using the Delphi technique’. EFSA Support Publ 13:1–139 https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/sp.efsa.2016.EN-1007
Bartsch SM, O'Shea KJ, Lee BY (2020) The clinical and economic burden of norovirus gastroenteritis in the United States. J Infect Dis 222:1910–1919. https://doi.org/10.1093/infdis/jiaa292
Li D, Baert L, Uyttendaele M (2013) Inactivation of food-borne viruses using natural biochemical substances. Food Microbiol 35:1–9. https://doi.org/10.1016/j.fm.2013.02.009
doi: 10.1016/j.fm.2013.02.009
pubmed: 23628607
Bialonska D, Kasimsetty SG, Schrader KK, Ferreira D (2009) The effect of pomegranate (Punica granatum L.) byproducts and ellagitannins on the growth of human gut bacteria. J Agric Food Chem 57:8344–8349. https://doi.org/10.1021/jf901931b
Ismail T, Akhtar S, Riaz M, Ismail A (2014) Effect of pomegranate peel supplementation on nutritional, organoleptic and stability properties of cookies. Int J Food Sci Nutr 65:661–666. https://doi.org/10.3109/09637486.2014.908170
doi: 10.3109/09637486.2014.908170
pubmed: 24725173
Nair M, Saxena A, Kaur C (2018) Effect of chitosan and alginate based coatings enriched with pomegranate peel extract to extend the postharvest quality of guava (Psidium guajava L.). Food Chem 240:245–252. https://doi.org/10.1016/j.foodchem.2017.07.122
Nozohour Y, Golmohammadi R, Mirnejad R, Fartashvand M (2018) Antibacterial activity of pomegranate (Punica granatum L.) seed and peel alcoholic extracts on Staphylococcus aureus and Pseudomonas aeruginosa isolated from health centers. J Appl Biotechnol Rep 5:32–36. https://doi.org/10.29252/jabr.01.01.06
Moradi MT, Karimi A, Rafieian-kopaei M, Rabiei-Faradonbeh M, Momtaz H (2020) Pomegranate peel extract inhibits internalization and replication of the influenza virus: an in vitro study. Avicenna J Phytomed 10:143–151 PMID:32257886
Li D, Baert L, Xia M, Zhong W, Jiang X, Uyttendaele M (2012) Effects of a variety of food extracts and juices on the specific binding ability of norovirus GII.4 P particles. J Food Prot 75:1350–1354. https://doi.org/10.4315/0362-028X.JFP-12-002
doi: 10.4315/0362-028X.JFP-12-002
pubmed: 22980024
pmcid: 4069861
Knight A, Haines J, Stals A, Li D, Uyttendaele M, Knight A, Jaykus LA (2016) A systematic review of human norovirus survival reveals a greater persistence of human norovirus RT-qPCR signals compared to those of cultivable surrogate viruses. Int J Food Microbiol 216:40–49. https://doi.org/10.1016/j.ijfoodmicro.2015.08.015
doi: 10.1016/j.ijfoodmicro.2015.08.015
pubmed: 26398283
European Pharmacopoeia 8 (2014) Monographs on herbal drugs and herbal drug preparations. Council of Europe, Strasbourg
Radin D, D’Souza DH (2011) Simple and rapid detection of human Norovirus from produce using SYBR green I-based real-time RT-PCR. Food Environ Virol 3:121–129. https://doi.org/10.1007/s12560-011-9066-5
doi: 10.1007/s12560-011-9066-5
Živković I, Šavikin K, Zdunić G, Živković J, Bigović D, Menković N, Radin D (2018) Antiviral activity of medicinal plants extracts against food-borne norovirus. Lekovite Sirovine 38:1–4. https://doi.org/10.5937/leksir1838031Z
doi: 10.5937/leksir1838031Z
Gullon B, Pintado ME, Pérez-Álvarez J, Viuda-Martos M (2016) Assessment of polyphenolic profile and antibacterial activity of pomegranate peel (Punica granatum) flour obtained from co-product of juice extraction. Food Control 59:94–98. https://doi.org/10.1016/j.foodcont.2015.05.025
Hasnaoui N, Wathelet B, Jiménez-Araujo A (2014) Valorization of pomegranate peel from 12 cultivars: dietary fibre composition, antioxidant capacity and functional properties. Food Chem 160:196–203. https://doi.org/10.1016/j.foodchem.2014.03.089
doi: 10.1016/j.foodchem.2014.03.089
pubmed: 24799227
Li J, He X, Li M, Zhao W, Liu L, Kong X (2015) Chemical fingerprint and quantitative analysis for quality control of polyphenols extracted from pomegranate peel by HPLC. Food Chem 176:7–11. https://doi.org/10.1016/j.foodchem.2014.12.040
doi: 10.1016/j.foodchem.2014.12.040
pubmed: 25624199
Russo M, Fanali C, Tripodo G, Dugo P, Muleo R, Dugo L et al (2018) Analysis of phenolic compounds in different parts of pomegranate (Punica granatum) fruit by HPLC-PDA-ESI/MS and evaluation of their antioxidant activity: application to different Italian varieties. Anal Bioanal Chem 410:3507–3520. https://doi.org/10.1007/s00216-018-0854-8
Kamimoto M, Nakai Y, Tsuji T, Shimamoto T, Shimamoto T (2014) Antiviral effects of persimmon extract on human norovirus and its surrogate, bacteriophage MS2. J Food Sci 79:M941–M946. https://doi.org/10.1111/1750-3841.12462
doi: 10.1111/1750-3841.12462
pubmed: 24749830
Li D, Baert L, Zhang D, Xia M, Zhong W, Van Coillie E, Jiang X, Uyttendaele M (2012) Effect of grape seed extract on human norovirus GII.4 and murine norovirus 1 in viral suspensions, on stainless steel discs, and in lettuce wash water. Appl Environ Microbiol 78:7572–7578. https://doi.org/10.1128/AEM.01987-12
doi: 10.1128/AEM.01987-12
pubmed: 22904060
pmcid: 3485726
Joshi S, Howell AB, D’Souza DH (2017) Blueberry proanthocyanidins against human norovirus surrogates in model foods and under simulated gastric conditions. Food Microbiol 63:263–267. https://doi.org/10.1016/j.fm.2016.11.024
doi: 10.1016/j.fm.2016.11.024
pubmed: 28040178
Higginbotham KL, Burris KP, Zivanovic S, Davidson P, Stewart C (2014) Antimicrobial activity of Hibiscus sabdariffa aqueous extracts against Escherichia coli O157:H7 and Staphylococcus aureus in a microbiological medium and milk of various fat concentrations. J Food Prot 77:262–268. https://doi.org/10.4315/0362-028x.jfp-13-313
Vermaak I, Viljoen AM, Hamman JH, Van Vuuren SF (2009) The effect of simulated gastrointestinal conditions on the antimicrobial activity and chemical composition of indigenous south African plant extracts. S Afr J Bot 75:594–599. https://doi.org/10.1016/j.sajb.2009.02.180
doi: 10.1016/j.sajb.2009.02.180
Singh P, Tripathi MK, Yasir M, Khare R, Tripathi MK, Shrivastava R (2020) Potential inhibitors for SARS-CoV-2 and functional food components as nutritional supplement for COVID-19: a review. Plant Foods Hum Nutr 75:458–466. https://doi.org/10.1007/s11130-020-00861-9
doi: 10.1007/s11130-020-00861-9
pubmed: 33037564
Suručić R, Tubić B, Stojiljković MP, Djuric DM, Travar M, Grabež M, Šavikin K, Škrbić R (2021) Computational study of pomegranate peel extract polyphenols as potential inhibitors of SARS-CoV-2 virus internalization. Mol Cell Biochem 476:1179–1193. https://doi.org/10.1007/s11010-020-03981-7
doi: 10.1007/s11010-020-03981-7
pubmed: 33200379