Nanoscale 3D EM reconstructions reveal intrinsic mechanisms of structural diversity of chemical synapses.
3D EM
activity
circuit
diversity
hippocampus
neuron
neurotransmission
reconstruction
spine
synapse
wiring
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
06 04 2021
06 04 2021
Historique:
received:
26
10
2020
revised:
21
01
2021
accepted:
15
03
2021
entrez:
7
4
2021
pubmed:
8
4
2021
medline:
27
1
2022
Statut:
ppublish
Résumé
Chemical synapses of shared cellular origins have remarkably heterogeneous structures, but how this diversity is generated is unclear. Here, we use three-dimensional (3D) electron microscopy and artificial intelligence algorithms for image processing to reconstruct functional excitatory microcircuits in the mouse hippocampus and microcircuits in which neurotransmitter signaling is permanently suppressed with genetic tools throughout the lifespan. These nanoscale analyses reveal that experience is dispensable for morphogenesis of synapses with different geometric shapes and contents of membrane organelles and that arrangement of morphologically distinct connections in local networks is stochastic. Moreover, loss of activity increases the variability in sizes of opposed pre- and postsynaptic structures without disrupting their alignments, suggesting that inherently variable weights of naive connections become progressively matched with repetitive use. These results demonstrate that mechanisms for the structural diversity of neuronal synapses are intrinsic and provide insights into how circuits essential for memory storage assemble and integrate information.
Identifiants
pubmed: 33826888
pii: S2211-1247(21)00267-9
doi: 10.1016/j.celrep.2021.108953
pmc: PMC8354523
mid: NIHMS1691250
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
108953Subventions
Organisme : NIGMS NIH HHS
ID : R24 GM137200
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM117049
Pays : United States
Organisme : NINDS NIH HHS
ID : U24 NS120055
Pays : United States
Organisme : NIMH NIH HHS
ID : RF1 MH123224
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH118442
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS087026
Pays : United States
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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