Immortalization of shrimp lymphoid cells by hybridizing with the continuous cell line Sf9 leading to the development of 'PmLyO-Sf9 '.

Animals Cell Line / immunology Lymphocytes / immunology Penaeidae / immunology Sf9 Cells Spodoptera
Cell fusion and TEM Hybrid cell line Sf9 cells Shrimp cell culture

Journal

Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220

Informations de publication

Date de publication:
Jun 2021
Historique:
received: 17 11 2020
revised: 25 03 2021
accepted: 29 03 2021
pubmed: 8 4 2021
medline: 26 5 2021
entrez: 7 4 2021
Statut: ppublish

Résumé

Shrimp progressively gets more attention among marine invertebrates from researchers all over the world due to it being a healthy food as well as having economic importance. There were a lot of attempts to develop a continuous cell line from shrimp but none successful. In this context a novel hybrid cell line named 'PmLyO-Sf9' could be developed by fusing shrimp lymphoid organ cells with Sf9 cells after to metabolic blocking of Sf9 cells using puromycin and actinomycin D and effecting the fusion by way of PEG application. The cells are maintained and multiplied in a mixture of SCCM and TNM-FH having osmolality 550 mOsm kg

Identifiants

DOI: 10.1016/j.fsi.2021.03.023 PMID: 33826940
pubmed: 33826940
pii: S1050-4648(21)00088-7
doi: 10.1016/j.fsi.2021.03.023
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

196-207

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

B S Anoop (BS)

National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, 682016, India.

Jayesh Puthumana (J)

National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, 682016, India.

Cijo George Vazhappilly (CG)

National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, 682016, India.

Salini Kombiyil (S)

National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, 682016, India.

Rosamma Philip (R)

Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, 682016, India.

Anas Abdulaziz (A)

CSIR-National Institute of Oceanography, Regional Centre, Cochin, 682019, India.

Isaac Sarojini Bright Singh (IS)

National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, 682016, India. Electronic address: isbsingh@gmail.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Immortalization of shrimp lymphoid cells by...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Immortalization of shrimp lymphoid cells by...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Immortalization of shrimp lymphoid cells by...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Crustacea › Decapoda (crustacea) › Penaeidae › Immortalization of shrimp lymphoid cells by...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Cellules Sf9 › Immortalization of shrimp lymphoid cells by...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Lepidoptera › Papillons de nuit › Spodoptera › Immortalization of shrimp lymphoid cells by...