Cross-priming amplification targeting the 18S rRNA gene for the rapid diagnosis of Babesia bovis infection.

Animals Babesia bovis / isolation & purification Babesiosis / diagnosis Cattle Cattle Diseases / diagnosis Cross-Priming Nucleic Acid Amplification Techniques / methods RNA, Protozoan / analysis RNA, Ribosomal, 18S / analysis Sensitivity and Specificity
Babesia bovis Bovine babesiosis Cross-priming amplification Diagnosis

Journal

Ticks and tick-borne diseases
ISSN: 1877-9603
Titre abrégé: Ticks Tick Borne Dis
Pays: Netherlands
ID NLM: 101522599

Informations de publication

Date de publication:
07 2021
Historique:
received: 26 02 2020
revised: 17 03 2021
accepted: 18 03 2021
pubmed: 8 4 2021
medline: 23 9 2021
entrez: 7 4 2021
Statut: ppublish

Résumé

Babesia bovis is a known causative agent of bovine babesiosis and is widely distributed across China. Rapid detection and accurate identification of B. bovis is essential for follow-up management and epidemiological investigations. In this study, a cross-priming amplification combined with vertical flow (CPA-VF) assay was developed. The detection limit of the CPA-VF assay targeting the 18S rRNA gene was 320 fg per reaction at 61 °C for 60 min. No cross-reactions were observed with other piroplasms infective to cattle. Furthermore, 36 blood samples from experimentally-infected animals were accurately assessed using the CPA-VF assay. The performance of the CPA-VF assay was compared with the results of conventional PCR for 219 blood samples from the field. Our results demonstrate that the CPA-VF assay is a practical and effective diagnostic tool for bovine babesiosis caused by B. bovis infection.

Identifiants

DOI: 10.1016/j.ttbdis.2021.101713 PMID: 33827035
pubmed: 33827035
pii: S1877-959X(21)00066-2
doi: 10.1016/j.ttbdis.2021.101713
pii:
doi:

Substances chimiques

RNA, Protozoan 0
RNA, Ribosomal, 18S 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101713

Informations de copyright

Copyright © 2021 Elsevier GmbH. All rights reserved.

Auteurs

Jinming Wang (J)

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: wjm0403@caas.cn.

Shandian Gao (S)

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: gaoshandian@caas.cn.

Jifei Yang (J)

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: yangjifei@caas.cn.

Junlong Liu (J)

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: liujunlong@caas.cn.

Youquan Li (Y)

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: liyouquan@caas.cn.

Jianxun Luo (J)

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: luojianxun@caas.cn.

Guiquan Guan (G)

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: guanguiquan@caas.cn.

Hong Yin (H)

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, PR China. Electronic address: yinhong@caas.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Eucaryotes › Alveolata › Apicomplexa › Piroplasmia › Piroplasmida › Babesia › Babesia bovis › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Maladies de l'animal › Parasitoses animales › Protozooses animales › Babésiose › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Maladies de l'animal › Maladies des bovins › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Phénomènes du système immunitaire › Activation des lymphocytes › Cross-priming › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN des protozoaires › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 18S › Cross-priming amplification targeting the 18S...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Cross-priming amplification targeting the 18S...