Crocetin suppresses the growth and migration in HCT-116 human colorectal cancer cells by activating the p-38 MAPK signaling pathway.
Crocetin
HCT-116 cells
Matrix metalloproteinase 9
Vascular endothelial growth factor
p38-mitogen activated protein kinase
Journal
Research in pharmaceutical sciences
ISSN: 1735-5362
Titre abrégé: Res Pharm Sci
Pays: Iran
ID NLM: 101516968
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
29
11
2019
revised:
09
05
2020
accepted:
14
11
2020
entrez:
8
4
2021
pubmed:
9
4
2021
medline:
9
4
2021
Statut:
epublish
Résumé
Crocetin is a natural antioxidant that is found in the crocus flower and HCT-116 cells were treated with different concentrations (0, 200, 400, 600, and 800 μM) of crocetin for 24 h. The cell survival rate was measured by MTT assay. Cell migration capacity was evaluated using the wound healing assay. The expression levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP-9) was monitored by RT-PCR. Phosphorylation of focal adhesion kinase (FAK) and p38 mitogen-activated protein kinase (MAPK) was determined using western blot. The proliferation of HCT-116 was inhibited by crocetin at 800 μM ( These data suggested that crocetin-induced growth- and migration- suppressing effects on HCT-116 cells may partially depend on the regulation of the p38 (MAPK) signaling pathway.
Sections du résumé
BACKGROUND AND PURPOSE
OBJECTIVE
Crocetin is a natural antioxidant that is found in the crocus flower and
EXPERIMENTAL APPROACH
METHODS
HCT-116 cells were treated with different concentrations (0, 200, 400, 600, and 800 μM) of crocetin for 24 h. The cell survival rate was measured by MTT assay. Cell migration capacity was evaluated using the wound healing assay. The expression levels of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP-9) was monitored by RT-PCR. Phosphorylation of focal adhesion kinase (FAK) and p38 mitogen-activated protein kinase (MAPK) was determined using western blot.
FINDINGS/RESULTS
RESULTS
The proliferation of HCT-116 was inhibited by crocetin at 800 μM (
CONCLUSION AND IMPLICATION
CONCLUSIONS
These data suggested that crocetin-induced growth- and migration- suppressing effects on HCT-116 cells may partially depend on the regulation of the p38 (MAPK) signaling pathway.
Identifiants
pubmed: 33828602
doi: 10.4103/1735-5362.301344
pii: RPS-15-592
pmc: PMC8020854
doi:
Types de publication
Journal Article
Langues
eng
Pagination
592-601Informations de copyright
Copyright: © 2020 Research in Pharmaceutical Sciences.
Références
Cardiovasc Hematol Agents Med Chem. 2008 Oct;6(4):343-7
pubmed: 18855647
Genes Cancer. 2013 Sep;4(9-10):342-59
pubmed: 24349632
Cell. 2010 Apr 2;141(1):52-67
pubmed: 20371345
Cell. 2011 Oct 14;147(2):275-92
pubmed: 22000009
Phytother Res. 2012 Mar;26(3):381-6
pubmed: 21774008
Evid Based Complement Alternat Med. 2012;2012:378415
pubmed: 22454661
Exp Ther Med. 2017 Dec;14(6):5527-5534
pubmed: 29285087
J Cell Physiol. 2010 Sep;224(3):775-85
pubmed: 20432471
Biochim Biophys Acta. 2014 Jan;1845(1):20-30
pubmed: 24269582
Oncol Lett. 2018 May;15(5):7716-7724
pubmed: 29731901
Int J Mol Sci. 2015 Jan 09;16(1):1544-61
pubmed: 25584615
Res Pharm Sci. 2015 May-Jun;10(3):222-32
pubmed: 26600849
Pharmacol Ther. 2015 Feb;146:132-49
pubmed: 25316657
Curr Pharm Biotechnol. 2012 Jan;13(1):173-9
pubmed: 21466430
Front Biosci (Landmark Ed). 2014 Jan 01;19:687-706
pubmed: 24389213
Mol Carcinog. 2008 Jun;47(6):415-23
pubmed: 18058803
BMC Pharmacol. 2002 Mar 15;2:7
pubmed: 11914135
Front Biosci. 2008 May 01;13:3581-93
pubmed: 18508457
Oncol Lett. 2015 Mar;9(3):1254-1260
pubmed: 25663893
Cell Physiol Biochem. 2012;30(3):758-70
pubmed: 22890153
Saudi J Biol Sci. 2018 Dec;25(8):1767-1771
pubmed: 30591798
Food Chem Toxicol. 2015 Oct;84:125-32
pubmed: 26303273
J Cell Physiol. 2018 Mar;233(3):2170-2182
pubmed: 28407293
Anticancer Res. 2009 Nov;29(11):4439-49
pubmed: 20032390
Biochem Cell Biol. 2014 Apr;92(2):105-11
pubmed: 24697694
Toxicol Sci. 2007 Nov;100(1):109-17
pubmed: 17698513
Toxicol Appl Pharmacol. 2014 Dec 1;281(2):166-73
pubmed: 25111127
Immune Netw. 2016 Apr;16(2):85-98
pubmed: 27162525
Anticancer Res. 2016 Nov;36(11):5989-5997
pubmed: 27793925
J Ethnopharmacol. 2006 Aug 11;107(1):25-31
pubmed: 16529888
Biomed Pharmacother. 2018 Jun;102:1-8
pubmed: 29547743
Food Chem Toxicol. 2013 Jun;56:204-13
pubmed: 23454149
J Behav Med. 2014 Oct;37(5):1019-29
pubmed: 24027014
Biochem Cell Biol. 2013 Dec;91(6):397-403
pubmed: 24219281
Trends Mol Med. 2004 Mar;10(3):125-9
pubmed: 15102355
Res Pharm Sci. 2016 Mar-Apr;11(2):120-9
pubmed: 27168751
Onco Targets Ther. 2010 Jun 24;3:83-97
pubmed: 20616959
EXCLI J. 2019 Oct 21;18:936-949
pubmed: 31762720
Planta Med. 2011 Jan;77(2):146-51
pubmed: 20803418
Int J Biol Macromol. 2019 Jan;121:734-742
pubmed: 30342943
Oncol Lett. 2018 May;15(5):7603-7610
pubmed: 29849796
Mar Drugs. 2014 Sep 12;12(9):4783-98
pubmed: 25222667
J Cell Biochem. 2001;83(4):631-42
pubmed: 11746506
Food Chem Toxicol. 2018 Jun;116(Pt B):70-76
pubmed: 29630948