Detection and genomic characterization of hepatitis E virus genotype 3 from pigs in Ghana, Africa.
Foodborne diseases
Infectious disease reservoirs
Livestock
One health
Viral hepatitis
Zoonoses
Journal
One health outlook
ISSN: 2524-4655
Titre abrégé: One Health Outlook
Pays: England
ID NLM: 101769253
Informations de publication
Date de publication:
2020
2020
Historique:
received:
10
01
2020
accepted:
05
05
2020
entrez:
8
4
2021
pubmed:
9
4
2021
medline:
9
4
2021
Statut:
epublish
Résumé
Hepatitis E virus (HEV) is a major cause of human hepatitis worldwide. Zoonotic genotypes of the virus have been found in diverse animal species with pigs playing a major role. Putative risk of zoonotic infection from livestock particularly swine in Sub-Saharan Africa including Ghana is poorly understood due to scarcity of available data, especially HEV sequence information. Serum samples were collected from cattle, sheep, goats and pigs from Kumasi in the Ashanti region of Ghana. Samples were subjected to nested RT-PCR screening and quantification of HEV RNA-positive samples using real-time RT-PCR and the World Health Organization International Standard for HEV. Testing of all pig samples for antibodies was done by ELISA. Sanger sequencing and genotyping was performed and one representative complete genome was generated to facilitate genome-wide comparison to other available African HEV sequences by phylogenetic analysis. A total of 420 samples were available from cattle ( HEV genotype 3 is highly endemic in pigs in Ghana and likely poses a zoonotic risk to people exposed to pigs. HEV genotype 3 in Ghana shares a common origin with other virus strains from Sub-Saharan Africa.
Sections du résumé
BACKGROUND
BACKGROUND
Hepatitis E virus (HEV) is a major cause of human hepatitis worldwide. Zoonotic genotypes of the virus have been found in diverse animal species with pigs playing a major role. Putative risk of zoonotic infection from livestock particularly swine in Sub-Saharan Africa including Ghana is poorly understood due to scarcity of available data, especially HEV sequence information.
METHODS
METHODS
Serum samples were collected from cattle, sheep, goats and pigs from Kumasi in the Ashanti region of Ghana. Samples were subjected to nested RT-PCR screening and quantification of HEV RNA-positive samples using real-time RT-PCR and the World Health Organization International Standard for HEV. Testing of all pig samples for antibodies was done by ELISA. Sanger sequencing and genotyping was performed and one representative complete genome was generated to facilitate genome-wide comparison to other available African HEV sequences by phylogenetic analysis.
RESULTS
RESULTS
A total of 420 samples were available from cattle (
CONCLUSION
CONCLUSIONS
HEV genotype 3 is highly endemic in pigs in Ghana and likely poses a zoonotic risk to people exposed to pigs. HEV genotype 3 in Ghana shares a common origin with other virus strains from Sub-Saharan Africa.
Identifiants
pubmed: 33829131
doi: 10.1186/s42522-020-00018-3
pii: 18
pmc: PMC7993477
doi:
Types de publication
Journal Article
Langues
eng
Pagination
10Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no competing interests.
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