Aberrant expression of cytokines in polycythemia vera correlate with the risk of thrombosis.
Cytokines
Inflammation
Myeloproliferative neoplasm
Polycythemia vera
Thrombosis
Journal
Blood cells, molecules & diseases
ISSN: 1096-0961
Titre abrégé: Blood Cells Mol Dis
Pays: United States
ID NLM: 9509932
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
24
02
2021
revised:
23
03
2021
accepted:
24
03
2021
pubmed:
9
4
2021
medline:
10
8
2021
entrez:
8
4
2021
Statut:
ppublish
Résumé
Thrombo-hemorrhagic complications cause significant morbidity and mortality in patients with polycythemia vera (PV). To assay and correlate inflammatory cytokines with the thrombotic risk in PV patients. This prospective observational study was carried out at Postgraduate Institute of Medical Education and Research, Chandigarh, India over 18-months. The study enrolled 52 patients with PV (newly diagnosed = 28, follow-up = 24), and 20 age/sex-matched controls. Cytokine analysis for IL 1β, IL2, IL4, IL6, IL8, IL10, IL11, IL12/23p40, TNFα, and IFN-γ was performed on the peripheral blood (before treatment initiation for newly diagnosed cases, and after 7 days of stopping drugs for follow-up cases) by flow cytometry-based cytokine bead analysis (CBA) using CBA kits (BD™ biosciences, USA). Results were analyzed using SPSS Statistics 22.0. The mean age of patients was 51.9 ± 13 years. Levels of IL-6, IL-1β, IL-8, IL-11, IL-12/23p40 were significantly raised, however, TNF-α, and IFN-γ levels were significantly lower in the PV population as compared to controls. A significant correlation between the levels of IL-6, IL-2, and IL-8 with the overall risk of thrombosis in PV patients was observed. PV patients display an aberrant pattern of plasma cytokine expression, the levels of which correlate with the thrombotic risk.
Sections du résumé
BACKGROUND
Thrombo-hemorrhagic complications cause significant morbidity and mortality in patients with polycythemia vera (PV).
OBJECTIVES
To assay and correlate inflammatory cytokines with the thrombotic risk in PV patients.
METHODS
This prospective observational study was carried out at Postgraduate Institute of Medical Education and Research, Chandigarh, India over 18-months. The study enrolled 52 patients with PV (newly diagnosed = 28, follow-up = 24), and 20 age/sex-matched controls. Cytokine analysis for IL 1β, IL2, IL4, IL6, IL8, IL10, IL11, IL12/23p40, TNFα, and IFN-γ was performed on the peripheral blood (before treatment initiation for newly diagnosed cases, and after 7 days of stopping drugs for follow-up cases) by flow cytometry-based cytokine bead analysis (CBA) using CBA kits (BD™ biosciences, USA). Results were analyzed using SPSS Statistics 22.0.
RESULTS
The mean age of patients was 51.9 ± 13 years. Levels of IL-6, IL-1β, IL-8, IL-11, IL-12/23p40 were significantly raised, however, TNF-α, and IFN-γ levels were significantly lower in the PV population as compared to controls. A significant correlation between the levels of IL-6, IL-2, and IL-8 with the overall risk of thrombosis in PV patients was observed.
CONCLUSIONS
PV patients display an aberrant pattern of plasma cytokine expression, the levels of which correlate with the thrombotic risk.
Identifiants
pubmed: 33831662
pii: S1079-9796(21)00031-0
doi: 10.1016/j.bcmd.2021.102565
pii:
doi:
Substances chimiques
Cytokines
0
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
102565Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.