Development of multiplex real-time PCR assays for differential detection of capripoxvirus, parapoxvirus and foot-and-mouth disease virus.
Animals
Capripoxvirus
/ genetics
Cattle
Communicable Diseases
/ veterinary
Foot-and-Mouth Disease
/ diagnosis
Foot-and-Mouth Disease Virus
/ genetics
Goat Diseases
/ diagnosis
Parapoxvirus
/ genetics
Poxviridae Infections
/ diagnosis
Real-Time Polymerase Chain Reaction
/ veterinary
Sensitivity and Specificity
Sheep
capripoxvirus
differential detection
foot-and-mouth disease virus
multiplex real-time PCR
parapoxvirus
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
revised:
30
03
2021
received:
15
09
2020
accepted:
01
04
2021
pubmed:
11
4
2021
medline:
7
5
2022
entrez:
10
4
2021
Statut:
ppublish
Résumé
This study reports the development of multiplex real-time PCR assays for differential detection of capripoxvirus (CaPV), parapoxvirus (PaPV) and foot-and-mouth disease virus (FMDV) in sheep, goats and cattle. Three multiplex assays were developed, a capripox (CaP) rule-out assay for simultaneous detection and differentiation of CaPV and PaPV, a FMD rule-out assay for simultaneous detection and differentiation of FMDV and PaPV, and a FMD/CaP rule-out assay for simultaneous detection and differentiation of CaPV, PaPV and FMDV. All multiplex assays included β-actin gene ACTB as an internal positive control to monitor PCR inhibition and accuracy of nucleic acid extractions. The optimized assays were highly specific to the target viruses (CaPV, PaPV and FMDV) with no cross-reactivity against other viruses that cause similar clinical signs. Using positive control plasmids as template, the limit of detection (LOD) of the multiplex assays were estimated as 2 CaPV, 7 PaPV and 15 FMDV copies per assay. The amplification efficiency (AE) and correlation coefficient (R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1326-1337Informations de copyright
© 2021 Wiley-VCH GmbH.
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