An Antibacterial Nanorobotic Approach for the Specific Targeting and Removal of Multiple Drug-Resistant Staphylococcus aureus.
USA300
ab-nanobot
antimicrobial resistance
electromagnetic-stimulation
mice subcutaneous skin infection model
radiofrequency
staphylococcus aureus
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
revised:
06
03
2021
received:
14
01
2021
pubmed:
11
4
2021
medline:
27
10
2021
entrez:
10
4
2021
Statut:
ppublish
Résumé
Methicillin-resistant Staphylococcus aureus (MRSA) causes diseases ranging from skin infections to lethal sepsis and has become a serious threat to human health due to multiple-drug resistance (MDR). Therefore, a resistance-free antibacterial therapy is necessary to overcome MDR MRSA infections. In this study, an antibacterial nanorobot (Ab-nanobot) is developed wherein a cell wall-binding domain (CBD)-endolysin, acting as a sensor, is covalently conjugated with an actuator consisting of an iron oxide/silica core-shell. The CBD-endolysin sensor shows an excellent specificity to detect, bind, and accumulate on the S. aureus USA300 cell surface even in a bacterial consortium, and in host cell infections. Ab-nanobot specifically captures and kills MRSA in response to medically approved radiofrequency (RF) electromagnetic stimulation (EMS) signal. When Ab-nanobot receives the RF-EMS signal on the cell surface, actuator induces cell death in MRSA with 99.999% removal within 20 min by cell-wall damage via generation of localized heat and reactive oxygen species. The in vivo efficacy of Ab-nanobot is proven using a mice subcutaneous skin infection model. Collectively, this study offers a nanomedical resistance-free strategy to overcome MDR MRSA infections by providing a highly specific nanorobot for S. aureus.
Identifiants
pubmed: 33838013
doi: 10.1002/smll.202100257
doi:
Substances chimiques
Anti-Bacterial Agents
0
Pharmaceutical Preparations
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2100257Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
M. Z. David, R. S. Daum, Clin. Microbiol. Rev. 2010, 23, 616.
C. Bouvet, S. Gjoni, B. Zenelaj, B. A. Lipsky, E. Hakko, I. Uckay, Int. J. Infect. Dis. 2017, 60, 44.
M. H. Kollef, A. Shorr, Y. P. Tabak, V. Gupta, L. Z. Liu, R. S. Johannes, Chest 2005, 128, 3854.
G. R. Corey, Clin. Infect. Dis. 2009, 48, S254.
Y. Guo, G. Song, M. Sun, J. Wang, Y. Wang, Front. Cell. Infect. Microbiol. 2020, 10, 107.
F. Peyrusson, H. Varet, T. K. Nguyen, R. Legendre, O. Sismeiro, J. Y. Coppee, C. Wolz, T. Tenson, F. Van Bambeke, Nat. Commun. 2020, 11, 2200.
A. C. Juayang, G. B. de Los Reyes, A. J. de la Rama, C. T. Gallega, Interdiscip. Perspect. Infect. Dis. 2014, 2014, 1.
M. Mahlapuu, J. Håkansson, L. Ringstad, C. Björn, Front. Cell. Infect. Microbiol. 2016, 6, 194.
A. Pai, G. Sudhakar, V. Kamath, Int. J. Pharm. Res. 2013, 3, 69.
P. Mizar, R. Arya, T. Kim, S. Cha, K. S. Ryu, W. S. Yeo, T. Bae, W. Kim, K. H. Park, K. K. Kim, S. S. Lee, J. Med. Chem. 2018, 61, 10473.
T. A. Hamilton, G. M. Pellegrino, J. A. Therrien, D. T. Ham, P. C. Bartlett, B. J. Karas, G. B. Gloor, D. R. Edgell, Nat. Commun. 2019, 10, 4544.
F. d'Herelle, G. H. Smith, Am. Assoc. Immnol. 1926, 1.
E. Malka, I. Perelshtein, A. Lipovsky, Y. Shalom, L. Naparstek, N. Perkas, T. Patick, R. Lubart, Y. Nitzan, E. Banin, A. Gedanken, Small 2013, 9, 4069.
E. N. Taylor, K. M. Kummer, N. G. Durmus, K. Leuba, K. M. Tarquinio, T. J. Webster, Small 2012, 8, 3016.
J. Z. Kubicek-Sutherland, H. Lofton, M. Vestergaard, K. Hjort, H. Ingmer, D. I. Andersson, J. Antimicrob. Chemother. 2017, 72, 115.
N. Principi, E. Silvestri, S. Esposito, Front. Pharmacol. 2019, 10, 513.
T. Maeda, R. García-Contreras, M. Pu, L. Sheng, L. R. Garcia, M. Tomás, T. K. Wood, ISME J. 2012, 6, 493.
E. Pursey, D. Sunderhauf, W. H. Gaze, E. R. Westra, S. V. Houte, PLoS Pathog. 2018, 14, e1006990.
N. Y. Lee, W. C. Ko, P. R. Hsueh, Front. Pharmacol. 2019, 10, 1153.
Y. N. Slavin, J. Asnis, U. O. Hafeli, H. Bach, J. Nanobiotechnol. 2017, 15, 65.
A. Gupta, S. Mumtaz, C. H. Li, I. Hussain, V. M. Rotello, Chem. Soc. Rev. 2019, 48, 415.
G. R. Rudramurthy, M. K. Swamy, U. R. Sinniah, A. Ghasemzadeh, Molecules 2016, 21, 836.
N. Khlebtsov, L. Dykman, Chem. Soc. Rev. 2011, 40, 1647.
M. Salouti, A. Ahangari, Nanoparticle Based Drug Delivery Systems for Treatment of Infectious Diseases, Vol. 552, InTech, London, UK 2014, p. 155.
A. K. Chaurasia, N. D. Thorat, A. Tandon, J.-H. Kim, S. H. P. Kim, K. K. Kim, Sci. Rep. 2016, 6, 1.
S. S. Broendum, A. M. Buckle, S. McGowan, Mol. Microbiol. 2018, 110, 879.
Y. Chang, S. Ryu, Appl. Microbiol. Biotechnol. 2017, 101, 147.
B. Son, M. Kong, S. Ryu, Viruses 2018, 10, 284.
F. C. Tenover, R. V. Goering, J. Antimicrob. Chemother. 2009, 64, 441.
G. C. Lee, S. D. Dallas, Y. Wang, R. J. Olsen, K. A. Lawson, J. Wilson, C. R. Frei, J. Antimicrob. Chemother. 2017, 72, 2461.
R. Lotan, N. Sharon, D. Mirelman, Eur. J. Biochem. 1975, 55, 257.
E. Mine, A. Yamada, Y. Kobayashi, M. Konno, L. M. Liz-Marzan, J. Colloid Interface Sci. 2003, 264, 385.
B. M. Chang, L. Pan, H. H. Lin, H. C. Chang, Sci. Rep. 2019, 9, 13164.
H. L. Ding, Y. X. Zhang, S. Wang, J. M. Xu, S. C. Xu, G. H. Li, Chem. Mater. 2012, 24, 4572.
M. A. Ghauri, N. Okibe, D. B. Johnson, Hydrometallurgy 2007, 85, 72.
M. Arakha, S. Pal, D. Samantarrai, T. K. Panigrahi, B. C. Mallick, K. Pramanik, B. Mallick, S. Jha, Sci. Rep. 2015, 5, 14813.
Y. Z, Q. Li, J. Wang, Y. Yu, Y. Wang, Q. Zhou, P. Li, Nanoscale Res. Lett. 2020, 15, 115.
T. Deng, H. Zhao, M. Shi, Y. Qiu, S. Jiang, X. Yang, Y. Zhao, Y. Zhang, Small 2019, 15, 1902647.
L. K. Chipps, J. Bentow, H. B. Prather, J. J. So, J. M. Schouest, D. M. Ozog, R. L. Moy, J. Drugs Dermatol. 2013, 12, 1215.
M. Gorgu, A. Gokkaya, J. Kizilkan, E. Karanfil, A. Dogan, A. Turk, J. Plast. Surg. 2019, 27, 62.
B. I. Eisenstein, Clin. Microbiol. Infect. 2008, 14, 17.
T. Denayer, T. Stöhr, M. V. Roy, New Horiz. Transl. Med. 2014, 2, 5.
J. Park, K. An, Nat. Mater. 2004, 3, 891.
K. Kekalo, K. Koo, E. Zeitchick, I. Baker, Mater. Res. Soc. Symp. Proc. 2012, 1416, 1.
I. L. Hsiao, S. Fritsch-Decker, A. Leidner, M. Al-Rawi, V. Hug, S. Diabate, S. L. Grage, M. Meffert, T. Stoeger, D. Gerthsen, A. S. Ulrich, C. M. Niemeyer, C. Weiss, Small 2019, 15, 1805400.
A. K. Buell, D. A. White, C. Meier, M. E. Welland, T. P. Knowles, C. M. Dobson, J. Phys. Chem. B 2010, 114, 10925.
X. Yu, Y. Song, Y. Di, H. He, D. Fu, C. Jin, Sci. Rep. 2016, 6, 31539.
H. Possel, H. Noack, W. Augustin, G. Keilhoff, G. Wolf, FEBS Lett. 1997, 416, 175.
R. Haugland, Molecular Probes Handbook of Fluorescent Probes and Research Chemicals, 6th ed., Interchim (Molecular Probes), Eugene 1996.
A. H. Fischer, K. A. Jacobson, J. Rose, R. Zeller, CSH Protoc. 2008, 3, 1.