Toward a deeper and simpler understanding of serum protein-mediated transformations of magnetic nanoparticles by ICP-MS.
Human serum
ICP-MS
Magnetic nanoparticles
Magnetic separation
Metal-containing proteins
Protein corona
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Jul 2021
01 Jul 2021
Historique:
received:
25
01
2021
revised:
03
03
2021
accepted:
06
03
2021
entrez:
11
4
2021
pubmed:
12
4
2021
medline:
1
5
2021
Statut:
ppublish
Résumé
A great variety of magnetic nanomaterials are entering preclinical investigations with the objective to select the most promising candidates for diagnostic and therapeutic applications. For an analytical approach to be used as a high-throughput screening tool, simple and cost-efficient sample preparation protocol is a basiс prerequisite. Here, we demonstrate how the application of continuous magnetic field allows for quantitatively separating iron oxide magnetic nanoparticles from a mixture with human serum to facilitate monitoring of their biomolecular interactions with high-resolution inductively coupled plasma mass spectrometry. By measuring the signals of sulfur and metal isotopes, it is possible to monitor the formation of the protein corona and alterations in the concentrations of relevant metals due to binding of specific metalloproteins, respectively.
Identifiants
pubmed: 33838780
pii: S0039-9140(21)00208-3
doi: 10.1016/j.talanta.2021.122287
pii:
doi:
Substances chimiques
Blood Proteins
0
Magnetite Nanoparticles
0
Metalloproteins
0
Metals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122287Informations de copyright
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