A turn-on fluorescent PCNA sensor.

Fluorescence Fluorescent Dyes / chemical synthesis Humans Molecular Structure Peptides / chemical synthesis Proliferating Cell Nuclear Antigen / chemistry Spectrometry, Fluorescence
Fluorescence Peptide Proliferating Cell Nuclear Antigen (PCNA) Sensor Solvatochromic amino-acid

Journal

Bioorganic & medicinal chemistry letters
ISSN: 1464-3405
Titre abrégé: Bioorg Med Chem Lett
Pays: England
ID NLM: 9107377

Informations de publication

Date de publication:
01 06 2021
Historique:
received: 03 02 2021
revised: 31 03 2021
accepted: 05 04 2021
pubmed: 12 4 2021
medline: 18 9 2021
entrez: 11 4 2021
Statut: ppublish

Résumé

The solvatochromic amino-acids 4-DMNA or 4-DAPA, were separately introduced at position 147, 150 or 151 of a short p21 peptide (141-155) known to bind sliding clamp protein PCNA. The ability of these peptides, 1a-3a and 1b-3b, to act as a turn-on fluorescent sensor for PCNA was then investigated. The 4-DMNA-containing peptides (1a-3a) displayed up to a 40-fold difference in fluorescence between a polar (Tris buffer) and a hydrophobic solvent (dioxane with 5 mM 18-crown-6), while the 4-DAPA-containing peptides (1b-3b) displayed a significantly enhanced (300-fold) increase in fluorescence from Tris buffer to dioxane with 18-crown-6. SPR analysis of the peptides against PCNA revealed that the 151-substituted peptides 3a and 3b interacted specifically with PCNA, with K

Identifiants

DOI: 10.1016/j.bmcl.2021.128031 PMID: 33839250
pubmed: 33839250
pii: S0960-894X(21)00257-2
doi: 10.1016/j.bmcl.2021.128031
pii:
doi:

Substances chimiques

Fluorescent Dyes 0
PCNA protein, human 0
Peptides 0
Proliferating Cell Nuclear Antigen 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

128031

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Aimee J Horsfall (AJ)

ARC Centre of Excellence for Nanoscale BioPhotonics, Institute of Photonics and Advanced Sensing, School of Physical Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia.

Theresa Chav (T)

ARC Centre of Excellence for Nanoscale BioPhotonics, Institute of Photonics and Advanced Sensing, School of Physical Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia.

John B Bruning (JB)

Institute of Photonics and Advanced Sensing, School of Biological Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia.

Andrew D Abell (AD)

ARC Centre of Excellence for Nanoscale BioPhotonics, Institute of Photonics and Advanced Sensing, School of Physical Sciences, The University of Adelaide, Adelaide, South Australia 5005, Australia. Electronic address: andrew.abell@adelaide.edu.au.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiques › Phénomènes optiques › Fluorescence › A turn-on fluorescent PCNA sensor.
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › A turn-on fluorescent PCNA sensor.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A turn-on fluorescent PCNA sensor.
questionsmedicales.fr › Phénomènes chimiques › Structure moléculaire › A turn-on fluorescent PCNA sensor.
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › A turn-on fluorescent PCNA sensor.
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › Antigène nucléaire de prolifération cellulaire › A turn-on fluorescent PCNA sensor.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrométrie de fluorescence › A turn-on fluorescent PCNA sensor.