HPV DNA genotyping, HPV E6*I mRNA detection, and p16

Adult Aged Aged, 80 and over Alphapapillomavirus / genetics Belgium Cyclin-Dependent Kinase Inhibitor p16 DNA, Viral / genetics Female Genotype Head and Neck Neoplasms / therapy Humans Ki-67 Antigen Male Middle Aged Oncogene Proteins, Viral / genetics Papillomavirus Infections / virology RNA, Messenger / isolation & purification Staining and Labeling

Head and neck neoplasms Human papillomavirus Human papillomavirus DNA tests mRNA p16(INK4a)

Journal

Cancer epidemiology

ISSN: 1877-783X

Titre abrégé: Cancer Epidemiol

Pays: Netherlands

ID NLM: 101508793

Informations de publication

Date de publication:
06 2021

Historique:

received: 24 09 2020

accepted: 26 02 2021

pubmed: 12 4 2021

medline: 8 7 2021

entrez: 11 4 2021

Statut: ppublish

Résumé

The main risk factors for head and neck cancer (HNC) are tobacco and alcohol use. However, an important fraction of oropharyngeal cancer (OPC) is caused by human papillomaviruses (HPV), a subgroup with increasing incidence in several western countries. As part of the HPV-AHEAD study, we assessed the role of HPV infection in 772 archived tissue specimens of Belgian HNC patients: 455 laryngeal (LC), 106 oral cavity (OCC), 99 OPC, 76 hypopharyngeal (HC), and 36 unspecified parts of the head and neck. All specimens were tested for HPV DNA (21 genotypes); whereof all HPV DNA-positives, all HPV DNA-negative OPCs and a random subset of HPV DNA-negatives of the other HNC-sites were tested for the presence of type-specific HPV RNA and p16 The highest HPV DNA prevalence was observed in OPC (36.4 %), and was significantly lower (p < 0.001) in the other HNCs (OCC:7.5 %, LC:6.6 %). HPV16 was the most common HPV-genotype in all HNCs. Approximately 83.0 % of the HPV DNA-positive OPCs tested HPV RNA or p16-positive, compared to about 37.5 % and 44.0 % in OCC and LC, respectively. Estimation of the attributable fraction of an HPV infection in HNC was very similar for HPV RNA or p16 in addition to DNA-positivity; with 30 % for OPC, and 3 % for OCC and LC. Our study confirms the heterogeneity of HPV DNA prevalence across anatomical sites in HNC, with a predominance of HPV16 in all sites. The estimated proportion of HPV-driven HNC in Belgium, during the period 1980-2014, was 10 times higher in OPC compared to OCC and LC.

Sections du résumé

BACKGROUND

METHODS

As part of the HPV-AHEAD study, we assessed the role of HPV infection in 772 archived tissue specimens of Belgian HNC patients: 455 laryngeal (LC), 106 oral cavity (OCC), 99 OPC, 76 hypopharyngeal (HC), and 36 unspecified parts of the head and neck. All specimens were tested for HPV DNA (21 genotypes); whereof all HPV DNA-positives, all HPV DNA-negative OPCs and a random subset of HPV DNA-negatives of the other HNC-sites were tested for the presence of type-specific HPV RNA and p16

RESULTS

The highest HPV DNA prevalence was observed in OPC (36.4 %), and was significantly lower (p < 0.001) in the other HNCs (OCC:7.5 %, LC:6.6 %). HPV16 was the most common HPV-genotype in all HNCs. Approximately 83.0 % of the HPV DNA-positive OPCs tested HPV RNA or p16-positive, compared to about 37.5 % and 44.0 % in OCC and LC, respectively. Estimation of the attributable fraction of an HPV infection in HNC was very similar for HPV RNA or p16 in addition to DNA-positivity; with 30 % for OPC, and 3 % for OCC and LC.

CONCLUSION

Our study confirms the heterogeneity of HPV DNA prevalence across anatomical sites in HNC, with a predominance of HPV16 in all sites. The estimated proportion of HPV-driven HNC in Belgium, during the period 1980-2014, was 10 times higher in OPC compared to OCC and LC.

Identifiants

DOI: 10.1016/j.canep.2021.101925 PMID: 33839457

pubmed: 33839457

pii: S1877-7821(21)00042-4

doi: 10.1016/j.canep.2021.101925

pii:

doi:

Substances chimiques

Cyclin-Dependent Kinase Inhibitor p16 0

DNA, Viral 0

E6 protein, human papillomavirus type 1 0

Ki-67 Antigen 0

Oncogene Proteins, Viral 0

RNA, Messenger 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

Pagination

101925

Investigateurs

Christel Herold-Mende (C)

Gerhard Dyckhoff (G)

George Mosialos (G)

Fausto Chiesa (F)

Marta Tagliabue (M)

Mohssen Ansarin (M)

Heiner Boeing (H)

Xavier Castellsagué (X)

Silvia de Sanjosé (S)

Marisa Mena (M)

Francesc Xavier Bosch (FX)

Laia Alemany (L)

Pulikottil Okkuru Esmy (PO)

Rudrapatna S Jayshree (RS)

Kortikere S Sabitha (KS)

Ashok M Shenoy (AM)

Manavalan Vijayakumar (M)

Aruna S Chiwate (AS)

Ranjit V Thorat (RV)

Girish G Hublikar (GG)

Shashikant S Lakshetti (SS)

Bhagwan M Nene (BM)

Amal Ch Kataki (AC)

Ashok Kumar Das (A)

Subha Sankaran (S)

Kunnambath Ramadas (K)

Christine Carreira (C)

Sandrine McKay-Chopin (S)

Thara Somanathan (T)

Alfredo Zito (A)