An Improved Method for Isolating High-Quality RNA From Rat Bone at Room Temperature Without the Need for Specialized Equipment.
bone
bone research
rat
rna extraction
rnalater ice
Journal
Cureus
ISSN: 2168-8184
Titre abrégé: Cureus
Pays: United States
ID NLM: 101596737
Informations de publication
Date de publication:
10 Mar 2021
10 Mar 2021
Historique:
entrez:
12
4
2021
pubmed:
13
4
2021
medline:
13
4
2021
Statut:
epublish
Résumé
Research on bone diseases often requires investigation of bone gene expression. Isolating high-quality RNA is essential to obtain reliable and accurate gene expression data. In an effort to analyze the expression of genes related to osteoporosis in rat bones, we developed an improved method for extraction of high-quality RNA without the need for liquid nitrogen or specialized equipment. This method involved transitioning frozen bone tissues to a more pliable state with
Identifiants
pubmed: 33842176
doi: 10.7759/cureus.13806
pmc: PMC8027952
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e13806Informations de copyright
Copyright © 2021, Poutoglidou et al.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
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