High-Throughput and Automated Acoustic Trapping of Extracellular Vesicles to Identify microRNAs With Diagnostic Potential for Prostate Cancer.

Molecular profiling of extracellular vesicles (EVs) offers novel opportunities for diagnostic applications, but the current major obstacle for clinical translation is the lack of efficient, robust, and reproducible isolation methods. To bridge that gap, we developed a microfluidic, non-contact, and low-input volume compatible acoustic trapping technology for EV isolation that enabled downstream small RNA sequencing. In the current study, we have further automated the acoustic microfluidics-based EV enrichment technique that enables us to serially process 32 clinical samples per run. We utilized the system to enrich EVs from urine collected as the first morning void from 207 men referred to 10-core prostate biopsy performed the same day. Using automated acoustic trapping, we successfully enriched EVs from 199/207 samples (96%). After RNA extraction, size selection, and library preparation, a total of 173/199 samples (87%) provided sufficient materials for next-generation sequencing that generated an average of 2 × 10

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Copyright © 2021 Ku, Fredsøe, Sørensen, Borre, Evander, Laurell, Lilja and Ceder.

Déclaration de conflit d'intérêts

TL and HL are founders and own stock in AcouSort AB and ME owns stock in AcouSort AB. AcouSort AB is a spin-off company from Lund University that manufactures and markets instrumentation for acoustic trapping. Unrelated to the current study, HL holds patents on assays for intact PSA (Antibody, immunoassay and method for prostate cancer detection. U.S. Patent No. US 7,872,104 B2) and a patent for a statistical method to detect prostate cancer (Methods and Apparatuses For Predicting Risk Of Prostate Cancer And Prostate Gland Volume. U.S. Patent No. US. 9,672,329 B2) commercialized by OPKO Health. HL receives royalties from sales of the test and has stock in OPKO Health. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.