Effects of freezing and short-term fixation on muscle mass, volume, and density.
correction
ethanol
formalin
preparation
Journal
Anatomical record (Hoboken, N.J. : 2007)
ISSN: 1932-8494
Titre abrégé: Anat Rec (Hoboken)
Pays: United States
ID NLM: 101292775
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
revised:
03
03
2021
received:
04
01
2021
accepted:
05
03
2021
pubmed:
13
4
2021
medline:
1
4
2022
entrez:
12
4
2021
Statut:
ppublish
Résumé
Preventing postmortem deterioration of soft-tissues is an important requisite of anatomical research. In order to provide corrections for potential myological distortions, this study quantifies the acute effects of freezing, formalin fixation and ethanol storage using muscles from (n = 46) rabbits (Oryctolagus cuniculus). Bilateral dissections of specific muscles were performed and each side was assigned to a different preparation group (fresh, formalin fixation only, fixation followed by short duration ethanol storage, and freezing once or twice). We demonstrate that short-term freezing at -20C and thawing have no significant effect on muscle mass, volume, and density while short-term formalin fixation and ethanol storage significantly reduces mass and volume (density remains relatively constant.) Although freezing may have less of an effect on the gross morphometric characteristics of the musculature than ethanol storage, slow freezing damages muscle microanatomy, and therefore, faster freezing and other modes of preservation such as formalin fixation and ethanol storage may be preferable. Based on our results, we derived the following correction factors for each preparation: the mass of specimens stored in 70% ethanol should be multiplied by 1.69 to approximate fresh muscle mass, and specimens fixed in 10% formalin multiplied by 1.32. Although not significant, specimens frozen-once were slightly less massive and could be multiplied by 1.03 (frozen-twice ×1.09). The volumetric corrections are: ethanol 1.64; 10% formalin 1.32; frozen-once 1.03; frozen-twice 1.10. While the density of ethanol preserved specimens is slightly less than that of fresh ones (correction: 1.03), those preserved in formalin and frozen maintain nearly the same density.
Substances chimiques
Formaldehyde
1HG84L3525
Ethanol
3K9958V90M
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
199-208Subventions
Organisme : National Science Foundation
ID : BCS-14-40599
Organisme : National Science Foundation
ID : IOS-15-57125
Informations de copyright
© 2021 American Association for Anatomy.
Références
Al-Hassan, L. A., Bujawari, J., & El-Silini, O. (2000). The effect of some preservatives and freezing on certain body dimensions of two species of the family Mullidae collected from Benghazi waters, Libya. Acta Ichthyologica Et Piscatoria, 2, 127-135.
Anapol, F., & Barry, K. (1996). Fiber architecture of the extensors of the hindlimb in semiterrestrial and arboreal guenons. American Journal of Physical Anthropology, 99, 429-447.
Andrei, M. A. & Genoways, H. H. (1999). Changes in pH in museum storage fluids. Collection Forum. pp. 63-75.
Añón, M. C., & Calvelo, A. (1980). Freezing rate effects on the drip loss of frozen beef. Meat Science, 4, 1-14.
Benjakul, S., & Bauer, F. (2000). Physicochemical and enzymatic changes of cod muscle proteins subjected to different freeze-thaw cycles. Journal of the Science of Food and Agriculture, 80, 1143-1150.
Billy, A. J. (1982). The effects of formalin and isopropyl alcohol on length and weight measurements of Sarotherodon mossambicus Trewavas. Journal of Fish Biology, 21, 107-112.
Birdseye, C. (1929). Some scientific aspects of packaging and quick-freezing perishable flesh products I-More rapid freezing means better preservation. Industrial and Engineering Chemistry, 21, 414-417.
Burgner, R. L. (1962). Studies of red salmon smolts from the Wood River Lakes, Alaska. Seattle, WA: University of Washington Press.
Callow, E. (1952). Frozen meat. Journal of the Science of Food and Agriculture, 3, 145-150.
Carroll, R., Cavanaugh, J., & Rorer, F. (1981). Effects of frozen storage on the ultrastructure of bovine muscle. Journal of Food Science, 46, 1091-1094.
Close, R. (1972). Dynamic properties of mammalian skeletal muscles. Physiological Reviews, 52, 129-197.
Cook, G., Love, E., Vickery, J., & Young, W. J. (1926). Studies on the refrigeration of meat. I. Investigations into the refrigeration of Beef. Australian Journal of Experimental Biology and Medical Science, 3, 15-31.
Cutts, A. (1988). Shrinkage of muscle fibres during the fixation of cadaveric tissue. Journal of Anatomy, 160, 75.
Deichmann, J. L., Boundy, J., & Williamson, G. B. (2009). Anuran artifacts of preservation: 27 years later. Phyllomedusa: Journal of Herpetology, 8, 51-58.
Eltoum, I., Fredenburgh, J., Myers, R. B., & Grizzle, W. E. (2001). Introduction to the theory and practice of fixation of tissues. Journal of Histotechnology, 24, 173-190.
Engel, S. (1974). Effects of formalin and freezing on length, weight and condition factor of cisco and yellow perch. Transactions of the American Fisheries Society, 103, 136-138.
Gans, C. (1982). Fiber architecture and muscle function. Exercise and Sport Sciences Reviews, 10, 160-207.
Gans, C., & Bock, W. J. (1965). The functional significance of muscle architecture-a theoretical analysis. Ergebnisse der Anatomie und Entwicklungsgeschichte, 38, 115-142.
Gans, C., & Gaunt, A. S. (1991). Muscle architecture in relation to function. Journal of Biomechanics, 24, 53-65.
Gonzalez-Sanguinetti, S., Anon, M., & Calvelo, A. (1985). Effect of thawing rate on the exudate production of frozen beef. Journal of Food Science, 50, 697-700.
Grujić, R., Petrović, L., Pikula, B., & Amidžić, L. (1993). Definition of the optimum freezing rate-1. Investigation of structure and ultrastructure of beef M. longissimus dorsi frozen at different freezing rates. Meat Science, 33, 301-318.
Hoar, W. S. (1939). The weight-length relationship of the Atlantic salmon. Journal of the Fisheries Board of Canada, 4, 441-460.
Jawad, L. A. (2003). The effect of formalin, alcohol and freezing on some body proportions of Alepes djeddaba (Pisces: Carangidae) collected from the Red Sea coast of Yemen. Revista de Biología Marina y Oceanografía, 38, 77-80.
Jones, D. (1976). Chemistry of fixation and preservation with aldehydes. In H. F. Steedman (Ed.), Zooplankton fixation and preservation (p. 155). Paris, France: UNESCO Press.
Kim, H.-W., Kim, J.-H., Seo, J.-K., Setyabrata, D., & Kim, Y. H. B. (2018). Effects of aging/freezing sequence and freezing rate on meat quality and oxidative stability of pork loins. Meat Science, 139, 162-170.
Klauber, L. M. (1943). Tail-length differences in snakes: With notes on sexual dimorphism and the coefficient of divergence [and] a graphic method of showing relationships. San Diego, CA: Zoological Society of San Diego.
Klemm, W. (1990). Dehydration: A new alcohol theory. Alcohol, 7, 49-59.
Lee, J. C. (1982). Accuracy and precision in anuran morphometrics: Artifacts of preservation. Systematic Biology, 31, 266-281.
Leonard, K. C., Worden, N., Boettcher, M., Dickinson, E., Omstead, K. M., Burrows, A., & Hartstone-Rose, A. (2021). Anatomical and ontogenetic influences on muscle density. Scientific Reports, 11, 1-11.
Leonard, K. C., Worden, N., Boettcher, M. L., Dickinson, E., & Hartstone-Rose, A. (2021). Effects of long-term ethanol storage on muscle architecture. The Anatomical Record.
Leygonie, C., Britz, T. J., & Hoffman, L. C. (2012). Impact of freezing and thawing on the quality of meat. Meat Science, 91, 93-98.
Lieber, R. L., & Friden, J. (2000). Functional and clinical significance of skeletal muscle architecture. Muscle & Nerve, 23, 1647-1666.
Luyet, B. J. (1964). Effects of freezing on muscle tissue. Proceedings of 17th Reciprocal Meat Conference.
Méndez, J., & Keys, A. (1960). Density and composition of mammalian muscle. Metabolism: Clinical and Experimental, 9, 184-188.
Mills, A. (1975). Measuring changes that occur during frozen storage of fish: A review. Journal of Food Technology, 10, 483-496.
Murphy, R. A., & Beardsley, A. C. (1974). Mechanical properties of the cat soleus muscle in situ. The American Journal of Physiology, 227, 1008-1013.
Ngapo, T., Babare, I., Reynolds, J., & Mawson, R. (1999). Freezing rate and frozen storage effects on the ultrastructure of samples of pork. Meat Science, 53, 159-168.
Otten, E. (1988). Concepts and models of functional architecture in skeletal muscle. Exercise and Sport Sciences Reviews, 16, 89-138.
Petrović, L., Grujić, R., & Petrović, M. (1993). Definition of the optimal freezing rate-2. Investigation of the physico-chemical properties of beef M. longissimus dorsi frozen at different freezing rates. Meat Science, 33, 319-331.
PubChem 2004. National Center for Biotechnology Information. Bethesda (MD): National Library of Medicine (US). [Internet]. PubChem Substance Record for SID 24895887, Source: Sigma-Aldrich; (cited December 17, 2020). Available from https://pubchem.ncbi.nlm.nih.gov/substance/24895887
Qi, J., Li, C., Chen, Y., Gao, F., Xu, X., & Zhou, G. (2012). Changes in meat quality of ovine longissimus dorsi muscle in response to repeated freeze and thaw. Meat Science, 92, 619-626.
Rahelić, S., Puač, S., & Gawwad, A. H. (1985). Structure of beef Longissimus dorsi muscle frozen at various temperatures: Part 1-Histological changes in muscle frozen at −10, −22, −33, −78, −115 and −196°C. Meat Science, 14, 63-72.
Ramsbottom, J., & Koonz, C. (1939). Freezing temperature as related to drip of frozen-defrosted beef. Journal of Food Science, 4, 425-431.
Reed, R. N. (2001). Effects of museum preservation techniques on length and mass of snakes. Amphibia-Reptilia, 22, 488-491.
Schumacher, G.-H. (1961). Funktionelle morphologie der kaumuskulatur. Jena: G. Fischer.
Scott, N. J. & Aquino-Shuster, A. L. (1989). The effects of freezing on formalin preservation of specimens of frogs and snakes. Collection Forum. pp. 41-16.
Sen, A., & Sharma, N. (2004). Effect of freezing and thawing on the histology and ultrastructure of buffalo muscle. Asian Australasian Journal of Animal Sciences, 17, 1291-1295.
Simmons, J. E. (2014). Fluid preservation: A comprehensive reference. Lanham, MD: Rowman & Littlefield.
Simmons, N. B., & Voss, R. S. (2009). Collection, preparation, and fixation of specimens and tissues (2nd ed.). Baltimore, MD: Johns Hopkins University Press.
Strange, E. D. (1987). Quantitation and characterization of drip from Frozen-Thawed and refrigerated pork liver. Journal of Food Science, 52, 910-915.
Ward, S. R., & Lieber, R. L. (2005). Density and hydration of fresh and fixed human skeletal muscle. Journal of Biomechanics, 38, 2317-2320.
Xia, X., Kong, B., Liu, Q., & Liu, J. (2009). Physicochemical change and protein oxidation in porcine longissimus dorsi as influenced by different freeze-thaw cycles. Meat Science, 83, 239-245.