A Retention-Matching Strategy for Method Transfer in Supercritical Fluid Chromatography: Introducing the Isomolar Plot Approach.
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
27 04 2021
27 04 2021
Historique:
pubmed:
13
4
2021
medline:
13
4
2021
entrez:
12
4
2021
Statut:
ppublish
Résumé
A strategy to match any retention shifts due to increased or decreased pressure drop during supercritical fluid chromatography (SFC) method transfer is presented. The strategy relies on adjusting the co-solvent molarity without the need to adjust the back-pressure regulator. Exact matching can be obtained with minimal changes in separation selectivity. To accomplish this, we introduce the isomolar plot approach, which shows the variation in molar co-solvent concentration depending on the mass fraction of co-solvent, pressure, and temperature, here exemplified by CO
Identifiants
pubmed: 33844504
doi: 10.1021/acs.analchem.0c05142
pmc: PMC8153393
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6385-6393Références
J Chromatogr A. 2017 May 26;1499:165-173
pubmed: 28389095
J Chromatogr A. 2013 Apr 12;1285:148-58
pubmed: 23477796
J Chromatogr A. 2014 Dec 29;1374:254-260
pubmed: 25499060
Anal Chem. 2019 Jan 2;91(1):210-239
pubmed: 30406984
J Chromatogr A. 2015 Dec 18;1425:280-6
pubmed: 26615709
Anal Bioanal Chem. 2018 Oct;410(25):6441-6457
pubmed: 30051210
J Chromatogr A. 2015 Aug 7;1406:316-23
pubmed: 26116187
J Chromatogr A. 2013 Aug 30;1305:285-92
pubmed: 23891374
J Chromatogr A. 2014 Oct 31;1366:126-35
pubmed: 25267710
Anal Chem. 2018 Sep 18;90(18):10828-10836
pubmed: 30133261
Anal Chem. 2020 Dec 1;92(23):15429-15436
pubmed: 33170667
J Chromatogr A. 2016 Apr 15;1442:129-39
pubmed: 26979267
J Chromatogr A. 2014 Oct 3;1362:278-93
pubmed: 25200534
J Chromatogr A. 2005 Oct 21;1092(1):149-60
pubmed: 16188570
J Chromatogr A. 2015 Jul 3;1401:52-9
pubmed: 26003622
J Chromatogr A. 2013 Dec 13;1320:130-7
pubmed: 24210558
J Chromatogr A. 2018 Sep 21;1568:177-187
pubmed: 30072233
J Pharm Biomed Anal. 2016 Sep 10;129:273-281
pubmed: 27442889
J Chromatogr A. 2017 Jan 27;1482:65-75
pubmed: 28057333
J Chromatogr A. 2016 Aug 12;1459:129-135
pubmed: 27401813
J Chromatogr A. 2014 Oct 3;1362:206-17
pubmed: 25189333