Local Delivery of Therapeutic Boron for Bone Healing Enhancement.
Journal
Journal of orthopaedic trauma
ISSN: 1531-2291
Titre abrégé: J Orthop Trauma
Pays: United States
ID NLM: 8807705
Informations de publication
Date de publication:
01 May 2021
01 May 2021
Historique:
accepted:
15
09
2020
entrez:
12
4
2021
pubmed:
13
4
2021
medline:
22
6
2021
Statut:
ppublish
Résumé
To evaluate if local delivery of boron can accelerate bone healing and examine if the bioactive salt impacts the osteogenic response of bone-derived osteoclasts and osteoblasts by the regulation of the Wnt/β-catenin pathway. Bilateral femoral cortical defects were created in 32 skeletally mature C57 mice. On the experimental side, boric acid (8 mg/kg concentration) was injected locally, whereas on the control side, saline was used. Mice were euthanized at 7, 14, and 28 days. MicroCT was used to quantify bone regeneration at the defect. Histological staining for alkaline phosphatase and tartrate-resistant acid phosphatase was used to quantify osteoblast and osteoclast activity, respectively. Immunohistochemical antibodies, β-catenin, and CD34 were used to quantify active β-catenin levels and angiogenesis, respectively. The boron group exhibited higher bone volume and trabecular thickness at 28 days on microCT. Both alkaline phosphatase activity and β-catenin activity was significantly higher in the boron group at 7 days. In addition, CD34 staining revealed increased angiogenesis at 14 days in boron-treated groups. We found boron to have no association with osteoclast activity. This study shows that local delivery of boron is associated with an increase in osteoblast activity at early phases of healing. The corresponding increase in β-catenin likely supports that boron increases osteoblast activity by the Wnt/β-catenin pathway. Increased angiogenesis at 14 days could be a separate mechanism of increasing bone formation that is independent of Wnt/β-catenin activation.
Identifiants
pubmed: 33844665
doi: 10.1097/BOT.0000000000001974
pii: 00005131-202105000-00014
doi:
Substances chimiques
Boron
N9E3X5056Q
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e165-e170Informations de copyright
Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.
Déclaration de conflit d'intérêts
The authors report no conflict of interest.
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