Membrane binding controls ordered self-assembly of animal septins.
Animals
Animals, Genetically Modified
Cell Membrane
/ metabolism
Cryoelectron Microscopy
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Humans
Lipid Bilayers
Membrane Lipids
/ chemistry
Microfilament Proteins
/ genetics
Microscopy, Atomic Force
Microscopy, Electron, Transmission
Protein Binding
Protein Conformation
Protein Multimerization
Quartz Crystal Microbalance Techniques
Septins
/ genetics
Structure-Activity Relationship
D. melanogaster
cell biology
cell surface mechanics
cortex
cytoskeleton
human
molecular biophysics
plasma membrane
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
13 04 2021
13 04 2021
Historique:
received:
22
09
2020
accepted:
12
04
2021
pubmed:
14
4
2021
medline:
27
10
2021
entrez:
13
4
2021
Statut:
epublish
Résumé
Septins are conserved cytoskeletal proteins that regulate cell cortex mechanics. The mechanisms of their interactions with the plasma membrane remain poorly understood. Here, we show by cell-free reconstitution that binding to flat lipid membranes requires electrostatic interactions of septins with anionic lipids and promotes the ordered self-assembly of fly septins into filamentous meshworks. Transmission electron microscopy reveals that both fly and mammalian septin hexamers form arrays of single and paired filaments. Atomic force microscopy and quartz crystal microbalance demonstrate that the fly filaments form mechanically rigid, 12- to 18-nm thick, double layers of septins. By contrast, C-terminally truncated septin mutants form 4-nm thin monolayers, indicating that stacking requires the C-terminal coiled coils on DSep2 and Pnut subunits. Our work shows that membrane binding is required for fly septins to form ordered arrays of single and paired filaments and provides new insights into the mechanisms by which septins may regulate cell surface mechanics.
Identifiants
pubmed: 33847563
doi: 10.7554/eLife.63349
pii: 63349
pmc: PMC8099429
doi:
pii:
Substances chimiques
Drosophila Proteins
0
Lipid Bilayers
0
Membrane Lipids
0
Microfilament Proteins
0
pnut protein, Drosophila
0
Septins
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R000174/1
Pays : United Kingdom
Informations de copyright
© 2021, Szuba et al.
Déclaration de conflit d'intérêts
AS, FB, GC, FI, MM, RR, AB, GK No competing interests declared
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