Human telomerase RNA component (hTERC) gene expression and chromosome 7 ploidy correlate positively with histological grade of cervical intraepithelial neoplasia.
Adult
Aged
Alphapapillomavirus
/ pathogenicity
Cervix Uteri
/ pathology
Chromosomes, Human, Pair 7
/ genetics
Cytodiagnosis
/ methods
DNA, Viral
/ genetics
Early Detection of Cancer
/ methods
Female
Gene Expression
/ genetics
Humans
Middle Aged
Papillomavirus Infections
/ diagnosis
Ploidies
RNA
/ genetics
Telomerase
/ genetics
Uterine Cervical Neoplasms
/ diagnosis
Young Adult
Uterine Cervical Dysplasia
/ diagnosis
Pap smear
biomarker
cervical cytology
cervical intraepithelial neoplasia
chromosome 7
hTERC
Journal
Cytopathology : official journal of the British Society for Clinical Cytology
ISSN: 1365-2303
Titre abrégé: Cytopathology
Pays: England
ID NLM: 9010345
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
27
02
2021
received:
25
05
2020
accepted:
17
03
2021
pubmed:
14
4
2021
medline:
19
1
2022
entrez:
13
4
2021
Statut:
ppublish
Résumé
Cervical cancer screening by primary human papilloma virus detection and cytology is fraught with low specificity and variable sensitivity, respectively. Cytology-histology correlation remains modest. Biomarkers associated with early genetic events in cervical squamous carcinogenesis and detectable in cytology material are likely to be relevant. Human telomerase RNA component (hTERC) gene overexpression and aneuploidy are promising candidates in view of their reported early and consistent association with cervical squamous oncogenesis. We analysed hTERC gene expression and chromosome 7 ploidy by fluorescent in-situ hybridisation (FISH) in 50 women with cytological precursor squamous intraepithelial lesions and available histology outcomes. Results were expressed as percentages of cells showing ≥3 signals, mean signals/nucleus, and maximum amplitude across various cytology and histology categories. Proportions of positive cases were calculated from threshold values derived from 6 controls. Distribution of above indices with respect to ≥cervical intraepithelial neoplasia 2 (CIN2) was explored. For both genetic aberrations, there was significant positive correlation (for all indices) between the proportion of positive cases and worsening cytological and histological outcomes (P < .05), with significant intergroup differences (P < .05). High-grade lesions (≥CIN2) had significantly higher results compared to <CIN2 lesions (P ≤ .001). In five discordant cases with ≥CIN2 under- or overdiagnosed on cytology, FISH supported the histological diagnosis. HTERC gene amplification and chromosome 7 ploidy showed positive association with cervical squamous carcinogenesis and could be relevant in settings of discrepant cytology-histology correlation.
Substances chimiques
DNA, Viral
0
telomerase RNA
0
RNA
63231-63-0
Telomerase
EC 2.7.7.49
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
631-639Informations de copyright
© 2021 John Wiley & Sons Ltd.
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