Emulating Early Atherosclerosis in a Vascular Microphysiological System Using Branched Tissue-Engineered Blood Vessels.
atherosclerosis
bifurcated arteries
microphysiological systems
pulsatile flows
tissue-engineered blood vessels
Journal
Advanced biology
ISSN: 2701-0198
Titre abrégé: Adv Biol (Weinh)
Pays: Germany
ID NLM: 101775319
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
revised:
26
01
2021
received:
20
10
2020
entrez:
14
4
2021
pubmed:
15
4
2021
medline:
26
10
2021
Statut:
ppublish
Résumé
Atherosclerosis begins with the accumulation of cholesterol-carrying lipoproteins on blood vessel walls and progresses to endothelial cell dysfunction, monocyte adhesion, and foam cell formation. Endothelialized tissue-engineered blood vessels (TEBVs) have previously been fabricated to recapitulate artery functionalities, including vasoconstriction, vasodilation, and endothelium activation. Here, the initiation of atherosclerosis is emulated by designing branched TEBVs (brTEBVs) of various geometries treated with enzyme-modified low-density-lipoprotein (eLDL) and TNF-α to induce endothelial cell dysfunction and adhesion of perfused human monocytes. Locations of monocyte adhesion under pulsatile flow are identified, and the hemodynamics in the brTEBVs are characterized using particle image velocimetry (PIV) and computational fluid dynamics (CFD). Monocyte adhesion is greater at the side outlets than at the main outlets or inlets, and is greatest at larger side outlet branching angles (60° or 80° vs 45°). In PIV experiments, the branched side outlets are identified as atherosclerosis-prone areas where fluorescent particles show a transient swirling motion following flow pulses; in CFD simulations, side outlets with larger branching angles show higher vorticity magnitude and greater flow disturbance than other areas. These results suggest that the branched TEBVs with eLDL/TNF-α treatment provide a physiologically relevant model of early atherosclerosis for preclinical studies.
Identifiants
pubmed: 33852179
doi: 10.1002/adbi.202000428
pmc: PMC9951769
mid: NIHMS1870359
doi:
Substances chimiques
Lipoproteins, LDL
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000428Subventions
Organisme : NCI NIH HHS
ID : P30 CA013696
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR025686
Pays : United States
Organisme : NCATS NIH HHS
ID : UH3 TR002142
Pays : United States
Organisme : NCATS NIH HHS
ID : UH3TR002142
Pays : United States
Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
PLoS One. 2017 Mar 27;12(3):e0174352
pubmed: 28346530
EuroIntervention. 2017 Oct 13;13(9):e1084-e1091
pubmed: 28606882
Nat Commun. 2017 Apr 06;8:14921
pubmed: 28382972
PLoS One. 2013 May 21;8(5):e63577
pubmed: 23704920
J Am Coll Cardiol. 2013 Oct 22;62(17):1541-51
pubmed: 23973684
Lab Chip. 2018 Jul 10;18(14):2047-2054
pubmed: 29927449
Stem Cell Reports. 2020 Feb 11;14(2):325-337
pubmed: 32032552
Stroke. 1985 Jul-Aug;16(4):582-7
pubmed: 2411027
Circulation. 1992 Jul;86(1):232-46
pubmed: 1535570
Biofabrication. 2020 Feb 27;12(2):025020
pubmed: 31739299
Sci Adv. 2020 Mar 25;6(13):eaaz2598
pubmed: 32232160
Biofabrication. 2020 Aug 10;12(4):045009
pubmed: 32650321
Biotechnol Prog. 2008 Jul-Aug;24(4):907-20
pubmed: 19194900
J Biomech Eng. 2002 Aug;124(4):397-407
pubmed: 12188206
Sci Rep. 2018 Aug 10;8(1):11954
pubmed: 30097618
Physiol Rev. 2004 Jul;84(3):767-801
pubmed: 15269336
Atherosclerosis. 2017 Jul;262:113-122
pubmed: 28535426
Sci Rep. 2016 Feb 18;6:21579
pubmed: 26888719
Ann Biomed Eng. 2010 Sep;38(9):2791-804
pubmed: 20387119
Sci Rep. 2015 Oct 12;5:15116
pubmed: 26456074
J Am Coll Cardiol. 2010 Apr 20;55(16):1679-87
pubmed: 20394871
Sci Rep. 2017 Aug 15;7(1):8168
pubmed: 28811655
J Atheroscler Thromb. 2019 Aug 1;26(8):720-727
pubmed: 30626781
Physiol Rev. 1995 Jul;75(3):487-517
pubmed: 7624392
Invest Radiol. 2006 Nov;41(11):793-8
pubmed: 17035869
Nat Rev Cardiol. 2019 Dec;16(12):727-744
pubmed: 31243391
Am J Physiol Cell Physiol. 2007 Dec;293(6):C1824-33
pubmed: 17913848
Int J High Throughput Screen. 2010 Oct 1;2010(1):171-181
pubmed: 21278926
Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14871-6
pubmed: 15466704
J Biomech. 2003 Dec;36(12):1883-95
pubmed: 14614942
Cardiovasc Res. 2015 Sep 1;107(4):556-67
pubmed: 26092101
Cardiovasc Res. 2014 Jul 1;103(1):37-46
pubmed: 24841070
Cardiovasc Res. 2018 Mar 15;114(4):590-600
pubmed: 29514202
Coron Artery Dis. 2000 Sep;11(6):495-502
pubmed: 10966136
Circ Res. 2007 Sep 28;101(7):723-33
pubmed: 17673673
Circ Cardiovasc Interv. 2011 Aug;4(4):355-61
pubmed: 21712525
Arterioscler Thromb Vasc Biol. 2016 Jun;36(6):1101-13
pubmed: 27079883
Am J Physiol Heart Circ Physiol. 2000 May;278(5):H1589-97
pubmed: 10775138
Biomed Eng Online. 2009 Oct 28;8:30
pubmed: 19863806
Biomaterials. 2016 Apr;85:180-194
pubmed: 26874281