Feline Calicivirus Proteinase-Polymerase Protein Degrades mRNAs To Inhibit Host Gene Expression.
Animals
Caliciviridae Infections
/ pathology
Calicivirus, Feline
/ genetics
Cats
Cell Line
HEK293 Cells
Humans
Immune Evasion
/ genetics
Peptide Hydrolases
/ genetics
Protein Biosynthesis
/ physiology
RNA Interference
RNA Stability
/ physiology
RNA, Messenger
/ metabolism
RNA, Small Interfering
/ genetics
Ribonucleases
/ genetics
Virus Replication
FCV
FCV PP
PP
mRNA degradation
ribonuclease
ribonuclease activity
shutoff
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
10 06 2021
10 06 2021
Historique:
pubmed:
16
4
2021
medline:
26
8
2021
entrez:
15
4
2021
Statut:
ppublish
Résumé
To replicate efficiently and evade the antiviral immune response of the host, some viruses degrade host mRNA to induce host gene shutoff via encoding shutoff factors. In this study, we found that feline calicivirus (FCV) infection promotes the degradation of endogenous and exogenous mRNAs and induces host gene shutoff, which results in global inhibition of host protein synthesis. Screening assays revealed that proteinase-polymerase (PP) is a most effective factor in reducing mRNA expression. Moreover, PP from differently virulent strains of FCV could induce mRNA degradation. Further, we found that the key sites of the PP protein required for its proteinase activity are also essential for its shutoff activity but also required for viral replication. The mechanism analysis showed that PP mainly targets Pol II-transcribed RNA in a ribosome-, 5' cap-, and 3' poly(A) tail-independent manner. Moreover, purified glutathione
Identifiants
pubmed: 33853967
pii: JVI.00336-21
doi: 10.1128/JVI.00336-21
pmc: PMC8437350
doi:
Substances chimiques
RNA, Messenger
0
RNA, Small Interfering
0
Ribonucleases
EC 3.1.-
Peptide Hydrolases
EC 3.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0033621Références
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