Detection and Quantification of African Swine Fever Virus in MA-104 Cells.
ASF
ASFV
African swine fever cell line
Diagnostics
MA-104
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Mar 2021
20 Mar 2021
Historique:
received:
08
09
2020
revised:
14
01
2021
accepted:
29
01
2021
entrez:
15
4
2021
pubmed:
16
4
2021
medline:
16
4
2021
Statut:
epublish
Résumé
Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1), a commercially available cell line isolated from African green monkey (
Identifiants
pubmed: 33855107
doi: 10.21769/BioProtoc.3955
pii: e3955
pmc: PMC8032485
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interests The authors Ayushi Rai, Manuel Borca and Douglas Gladue have filed a patent for using MA-104 cells as a substrate for detection of ASFV.
Références
Transbound Emerg Dis. 2020 Mar 7;:
pubmed: 32145150
Dev Biol Stand. 1985;60:125-31
pubmed: 4043530
Curr Top Microbiol Immunol. 2009;328:43-87
pubmed: 19216435
Viruses. 2020 Jul 28;12(8):
pubmed: 32731642
Sci Rep. 2018 Feb 16;8(1):3154
pubmed: 29453406