An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration.
Cell migration
High-throughput
Live cell image
Scratching assay
Time-lapse imaging
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Mar 2021
20 Mar 2021
Historique:
received:
23
09
2020
revised:
04
01
2021
accepted:
20
01
2021
entrez:
15
4
2021
pubmed:
16
4
2021
medline:
16
4
2021
Statut:
epublish
Résumé
In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even for practiced and adept experimenters, a high chance for variability and limited reproducibility. Therefore, our protocol implemented the assessment for cell mobility by using homogenous wound making, sequential imaging and automated image analysis. Cells were cultured in 96-well plates, and after attachment, homogeneous linear scratches were made using the IncuCyte
Identifiants
pubmed: 33855109
doi: 10.21769/BioProtoc.3957
pii: e3957
pmc: PMC8032497
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interests The authors declare that they have no conflicts of interest.
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