Orientation-Selective and Frequency-Correlated Light-Induced Pulsed Dipolar Spectroscopy.
Journal
The journal of physical chemistry letters
ISSN: 1948-7185
Titre abrégé: J Phys Chem Lett
Pays: United States
ID NLM: 101526034
Informations de publication
Date de publication:
22 Apr 2021
22 Apr 2021
Historique:
pubmed:
16
4
2021
medline:
16
4
2021
entrez:
15
4
2021
Statut:
ppublish
Résumé
We explore the potential of orientation-resolved pulsed dipolar spectroscopy (PDS) in light-induced versions of the experiment. The use of triplets as spin-active moieties for PDS offers an attractive tool for studying biochemical systems containing optically active cofactors. Cofactors are often rigidly bound within the protein structure, providing an accurate positional marker. The rigidity leads to orientation selection effects in PDS, which can be analyzed to give both distance and mutual orientation information. Herein we present a comprehensive analysis of the orientation selection of a full set of light-induced PDS experiments. We exploit the complementary information provided by the different light-induced techniques to yield atomic-level structural information. For the first time, we measure a 2D frequency-correlated laser-induced magnetic dipolar spectrum, and we are able to monitor the complete orientation dependence of the system in a single experiment. Alternatively, the summed spectrum enables an orientation-independent analysis to determine the distance distribution.
Identifiants
pubmed: 33856805
doi: 10.1021/acs.jpclett.1c00595
pmc: PMC8154851
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3819-3826Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103311
Pays : United States
Références
J Phys Chem A. 2008 Jun 12;112(23):5064-73
pubmed: 18491846
J Magn Reson. 2009 Nov;201(1):48-56
pubmed: 19758831
Chemphyschem. 2019 Apr 2;20(7):931-935
pubmed: 30817078
Angew Chem Int Ed Engl. 2017 Jan 2;56(1):177-181
pubmed: 27918126
J Am Chem Soc. 2007 Apr 25;129(16):4868-9
pubmed: 17394319
Acc Chem Res. 2014 Feb 18;47(2):688-95
pubmed: 24289139
J Phys Chem B. 2015 Oct 29;119(43):13534-42
pubmed: 26000868
Phys Chem Chem Phys. 2019 May 15;21(19):9810-9830
pubmed: 31025988
J Am Chem Soc. 1975 Apr 2;97(7):1688-94
pubmed: 166113
J Phys Chem Lett. 2016 Jun 16;7(12):2204-9
pubmed: 27163749
Biochemistry. 1989 Sep 19;28(19):7806-12
pubmed: 2558712
Sci Rep. 2017 Aug 8;7(1):7504
pubmed: 28790428
J Magn Reson. 2017 Jul;280:103-116
pubmed: 28579096
J Magn Reson. 2018 Nov;296:29-35
pubmed: 30199790
J Am Chem Soc. 2014 May 7;136(18):6582-5
pubmed: 24735449
J Phys Chem B. 2008 Jul 31;112(30):9079-90
pubmed: 18593147
Biochim Biophys Acta. 2014 Jan;1837(1):85-97
pubmed: 23871938
Dalton Trans. 2021 Jan 21;50(3):808-815
pubmed: 33416053
Biochim Biophys Acta. 2016 Dec;1857(12):1909-1916
pubmed: 27659505
Phys Chem Chem Phys. 2009 Aug 21;11(31):6840-8
pubmed: 19639159
Angew Chem Int Ed Engl. 2003 Jan 3;42(1):62-4
pubmed: 19757591
J Chem Phys. 2020 Jan 21;152(3):034201
pubmed: 31968969
Annu Rev Phys Chem. 2012;63:419-46
pubmed: 22404592
Chemistry. 2016 Nov 21;22(48):17204-17214
pubmed: 27868323
Free Radic Res. 2015 Jan;49(1):78-85
pubmed: 25348344
J Magn Reson. 2000 Feb;142(2):331-40
pubmed: 10648151
Front Mol Biosci. 2015 Sep 01;2:49
pubmed: 26389123
J Phys Chem Lett. 2016 Apr 21;7(8):1411-5
pubmed: 27035368
Angew Chem Int Ed Engl. 2019 Aug 19;58(34):11681-11685
pubmed: 31218813
Bioorg Med Chem. 1999 Jan;7(1):119-31
pubmed: 10199662
Angew Chem Int Ed Engl. 2015 May 18;54(21):6330-4
pubmed: 25821033
J Phys Chem Lett. 2021 Jan 14;12(1):80-85
pubmed: 33306382
J Photochem Photobiol B. 2020 Oct;211:112008
pubmed: 32932136
J Phys Chem B. 2018 Nov 29;122(47):10669-10677
pubmed: 30372072
Angew Chem Int Ed Engl. 2019 Sep 16;58(38):13271-13275
pubmed: 31322814
J Am Chem Soc. 2015 Jul 1;137(25):8284-93
pubmed: 26035477
J Am Chem Soc. 2002 Oct 23;124(42):12606-11
pubmed: 12381206
Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):1930-5
pubmed: 20133838
Methods Enzymol. 2015;563:459-81
pubmed: 26478495
J Am Chem Soc. 2018 Feb 21;140(7):2514-2527
pubmed: 29266939
Phys Chem Chem Phys. 2014 Jun 7;16(21):9685-99
pubmed: 24429839
Chem Commun (Camb). 2020 Dec 4;56(93):14669-14672
pubmed: 33159780
Q Rev Biophys. 2007 Feb;40(1):1-53
pubmed: 17565764
Chempluschem. 2020 Feb;85(2):353-372
pubmed: 31950648
Chemistry. 2019 Jul 2;25(37):8820-8828
pubmed: 31017706
Acc Chem Res. 2002 May;35(5):313-20
pubmed: 12020169
J Magn Reson. 2019 Sep;306:102-108
pubmed: 31337564
Angew Chem Int Ed Engl. 2016 Sep 12;55(38):11538-42
pubmed: 27511025
Biochim Biophys Acta. 2008 Oct;1777(10):1355-63
pubmed: 18602887
Phys Chem Chem Phys. 2016 Aug 17;18(33):23111-20
pubmed: 27491304
Emerg Top Life Sci. 2018 Apr 20;2(1):9-18
pubmed: 33525779
J Am Chem Soc. 2015 May 27;137(20):6670-9
pubmed: 25914154
Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13386-90
pubmed: 16938868
Phys Chem Chem Phys. 2013 Jan 14;15(2):619-27
pubmed: 23183777