Inflammatory Gene Expression of Human Perivascular Adipose Tissue in Abdominal Aortic Aneurysms.
Adipose Tissue
/ metabolism
Aged
Aged, 80 and over
Aortic Aneurysm, Abdominal
/ genetics
Case-Control Studies
Chemokine CCL5
/ genetics
Cytoskeletal Proteins
/ genetics
Female
Humans
Interleukin-8
/ genetics
Leukocyte Common Antigens
/ genetics
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
/ genetics
Male
Matrix Metalloproteinase 9
/ genetics
Middle Aged
Muscle Proteins
/ genetics
Myocytes, Smooth Muscle
/ metabolism
PPAR gamma
/ genetics
RNA, Messenger
/ metabolism
Abdominal aortic aneurysm
Human
Inflammation
Pathophysiology
Perivascular adipose tissue
Smooth muscle cells
Journal
European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery
ISSN: 1532-2165
Titre abrégé: Eur J Vasc Endovasc Surg
Pays: England
ID NLM: 9512728
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
13
04
2020
revised:
15
02
2021
accepted:
20
02
2021
pubmed:
17
4
2021
medline:
17
8
2021
entrez:
16
4
2021
Statut:
ppublish
Résumé
Perivascular adipose tissue (PVAT) contributes to vascular homeostasis and is increasingly linked to vascular pathology. PVAT density and volume were associated with abdominal aortic aneurysm (AAA) presence and dimensions on imaging. However, mechanisms underlying the role of PVAT in AAA have not been clarified. This study aimed to explore differences in PVAT from AAA using gene expression and functional tests. Human aortic PVAT and control subcutaneous adipose tissue were collected during open AAA surgery. Gene analyses and functional tests were performed. The control group consisted of healthy aorta from non-living renal transplant donors. Gene expression tests were performed to study genes potentially involved in various inflammatory processes and AAA related genes. Live PVAT and subcutaneous adipose tissue (SAT) from AAA were used for ex vivo co-culture with smooth muscle cells (SMCs) retrieved from non-pathological aortas. Adipose tissue was harvested from 27 AAA patients (n [gene expression] = 22, n [functional tests] = 5) and five control patients. An increased inflammatory gene expression of PTPRC (p = .008), CXCL8 (p = .033), LCK (p = .003), CCL5 (p = .004) and an increase in extracellular matrix breakdown marker MMP9 (p = .016) were found in AAA compared with controls. Also, there was a decreased anti-inflammatory gene expression of PPARG in AAA compared with controls (p = .040). SMC co-cultures from non-pathological aortas with PVAT from AAA showed increased MMP9 (p = .033) and SMTN (p = .008) expression and SAT increased SMTN expression in these SMC. The data revealed that PVAT from AAA shows an increased pro-inflammatory and matrix metallopeptidase gene expression and decreased anti-inflammatory gene expression. Furthermore, increased expression of genes involved in aneurysm formation was found in healthy SMC co-culture with PVAT of AAA patients. Therefore, PVAT from AAA might contribute to inflammation of the adjacent aortic wall and thereby plays a possible role in AAA pathophysiology. These proposed pathways of inflammatory induction could reveal new therapeutic targets in AAA treatment.
Identifiants
pubmed: 33858751
pii: S1078-5884(21)00186-6
doi: 10.1016/j.ejvs.2021.02.034
pii:
doi:
Substances chimiques
CCL5 protein, human
0
CXCL8 protein, human
0
Chemokine CCL5
0
Cytoskeletal Proteins
0
Interleukin-8
0
Muscle Proteins
0
PPAR gamma
0
PPARG protein, human
0
RNA, Messenger
0
SMTN protein, human
0
LCK protein, human
EC 2.7.10.2
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
EC 2.7.10.2
Leukocyte Common Antigens
EC 3.1.3.48
PTPRC protein, human
EC 3.1.3.48
MMP9 protein, human
EC 3.4.24.35
Matrix Metalloproteinase 9
EC 3.4.24.35
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1008-1016Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.