Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics.
Anti-Bacterial Agents
/ administration & dosage
Antineoplastic Agents
/ administration & dosage
Cell Communication
Cell Differentiation
Cells, Cultured
Chemokine CCL2
/ administration & dosage
Drug Delivery Systems
Extracellular Vesicles
Granulocyte Precursor Cells
/ cytology
Humans
Neutrophils
/ cytology
Paclitaxel
/ administration & dosage
Penicillins
/ administration & dosage
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 04 2021
15 04 2021
Historique:
received:
17
03
2020
accepted:
22
03
2021
entrez:
16
4
2021
pubmed:
17
4
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.
Identifiants
pubmed: 33859336
doi: 10.1038/s41598-021-87891-8
pii: 10.1038/s41598-021-87891-8
pmc: PMC8050327
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antineoplastic Agents
0
CCL2 protein, human
0
Chemokine CCL2
0
Penicillins
0
Paclitaxel
P88XT4IS4D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8289Références
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