16S amplicon sequencing of microbial communities in enriched and non-enriched sediments of non-volcanic hot spring with temperature gradients.
16S amplicon sequencing
Culture-dependent
Enrichment
Hot spring
Tatta Pani
Thermophile
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2021
2021
Historique:
received:
10
08
2020
accepted:
02
02
2021
entrez:
16
4
2021
pubmed:
17
4
2021
medline:
17
4
2021
Statut:
epublish
Résumé
Microorganisms in geothermal springs can offer insights into the fundamental and applied study of extremophiles. However, low microbial abundance and culturing requirements limit the ability to analyze microbial diversity in these ecosystems. In this study, culture-dependent and culture-independent techniques were used to analyze sediment samples from the non-volcanic Tatta Pani hot springs in district Poonch of Azad Kashmir. Microbial composition, temperature gradient, and enrichment effects on rare taxa were evaluated. In total, 31 distinct bacterial phyla and 725 genera were identified from the non-enriched Tatta Pani hot spring sediment samples, and 33 distinct bacterial phyla and 890 genera from the enriched sediment samples. Unique phyla specimens from the enriched samples included Candidatus Cloacimonetes, Caldiserica, and Korarchaeota archaea. The enriched samples yielded specific microbiota including 805 bacteria and 42 archaea operational taxonomic units with 97% similarity, though decreased thermophilic microbiota were observed in the enriched samples. Microbial diversity increased as temperature decreased. Candidate novel species were isolated from the culture-dependent screening, along with several genera that were not found in the 16S amplicon sequencing data. Overall, the enriched sediments showed high microbial diversity but with adverse changes in the composition of relatively dominant bacteria. Metagenomic analyses are needed to study the diversity, phylogeny, and functional investigation of hot spring microbiota.
Identifiants
pubmed: 33859871
doi: 10.7717/peerj.10995
pii: 10995
pmc: PMC8020870
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e10995Informations de copyright
© 2021 Yasir et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
Références
Arch Microbiol. 2018 Jan;200(1):1-18
pubmed: 28887679
ISME J. 2017 May;11(5):1158-1167
pubmed: 28072418
Front Microbiol. 2018 Sep 19;9:2096
pubmed: 30283408
ISME J. 2010 Dec;4(12):1485-95
pubmed: 20535223
Int J Syst Evol Microbiol. 2004 Jan;54(Pt 1):33-39
pubmed: 14742456
Astrobiology. 2019 Dec;19(12):1505-1522
pubmed: 31592688
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2263-2268
pubmed: 16280480
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):716-721
pubmed: 22140171
PLoS One. 2019 Oct 21;14(10):e0223543
pubmed: 31634343
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Extremophiles. 2016 Mar;20(2):207-14
pubmed: 26860120
Infect Drug Resist. 2019 Jun 21;12:1749-1761
pubmed: 31417292
Antonie Van Leeuwenhoek. 2016 Aug;109(8):1101-9
pubmed: 27170166
Sci Rep. 2019 Sep 5;9(1):12807
pubmed: 31488869
ISME J. 2017 Apr;11(4):853-862
pubmed: 28072420
Physiology (Bethesda). 2015 Mar;30(2):97-106
pubmed: 25729055
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:783-6
pubmed: 10319503
Clin Microbiol Infect. 2012 Dec;18(12):1185-93
pubmed: 23033984
Bioresour Technol. 2009 Oct;100(19):4396-403
pubmed: 19423335
Int J Syst Evol Microbiol. 2005 Sep;55(Pt 5):1877-1884
pubmed: 16166682
FEMS Microbiol Lett. 2010 Aug 1;309(1):1-7
pubmed: 20487025
Sci Rep. 2019 Feb 28;9(1):3059
pubmed: 30816235
Front Microbiol. 2013 May 06;4:67
pubmed: 23653623
PLoS One. 2010 Mar 19;5(3):e9773
pubmed: 20333304
Geobiology. 2018 Sep;16(5):507-521
pubmed: 29856116
BMC Bioinformatics. 2012 Feb 14;13:31
pubmed: 22333067
Front Microbiol. 2017 Jul 06;8:1252
pubmed: 28729863
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1609-13
pubmed: 7510403
OMICS. 2019 Jan;23(1):17-27
pubmed: 30589606
Enzyme Microb Technol. 2014 Apr 10;57:48-54
pubmed: 24629267
Sci Rep. 2019 Sep 17;9(1):13484
pubmed: 31530884
Int J Syst Evol Microbiol. 2008 Aug;58(Pt 8):1885-91
pubmed: 18676474
Environ Microbiol. 2019 Nov;21(11):4180-4195
pubmed: 31397054
Int J Syst Evol Microbiol. 2002 May;52(Pt 3):765-772
pubmed: 12054236
Int J Syst Evol Microbiol. 2001 Mar;51(Pt 2):481-488
pubmed: 11321094
Nutr Diabetes. 2015 Apr 27;5:e153
pubmed: 25915742
Nat Commun. 2018 Jul 23;9(1):2876
pubmed: 30038374
Extremophiles. 2017 Jan;21(1):135-152
pubmed: 27807621
Appl Environ Microbiol. 2009 Jul;75(13):4565-72
pubmed: 19429553
Int J Syst Evol Microbiol. 2016 Dec;66(12):5023-5027
pubmed: 27566409
PLoS One. 2012;7(6):e38108
pubmed: 22675512
Extremophiles. 2015 Nov;19(6):1067-76
pubmed: 26290358
Microb Ecol. 2017 Jul;74(1):116-127
pubmed: 28105510
Front Microbiol. 2020 Jul 14;11:1625
pubmed: 32760379
Archaea. 2013;2013:136714
pubmed: 23533327
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
BMC Microbiol. 2017 Sep 22;17(1):203
pubmed: 28938870
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):249-266
pubmed: 19700448
BMC Res Notes. 2014 Dec 09;7:892
pubmed: 25491533
Int J Syst Evol Microbiol. 2007 Jul;57(Pt 7):1429-1434
pubmed: 17625170