Adenosine Kinase Expression Determines DNA Methylation in Cancer Cell Lines.
Journal
ACS pharmacology & translational science
ISSN: 2575-9108
Titre abrégé: ACS Pharmacol Transl Sci
Pays: United States
ID NLM: 101721411
Informations de publication
Date de publication:
09 Apr 2021
09 Apr 2021
Historique:
received:
06
01
2021
entrez:
16
4
2021
pubmed:
17
4
2021
medline:
17
4
2021
Statut:
epublish
Résumé
DNA methylation has a major role in cancer, and its inhibitors are used therapeutically. DNA methylation depends on methyl group flux through the transmethylation pathway, which forms adenosine. We hypothesized that an adenosine kinase isoform with nuclear expression (ADK-L) determines global DNA methylation in cancer cells. We quantified ADK-L expression (Western Blot) and global DNA methylation as percent 5-methyldeoxycytidine (5mdC, LC-MS/MS) in three cancer lines (HeLa, HepG2, and U373). ADK-L expression and global DNA methylation correlated positively with the highest levels in HeLa cells compared to U373 and HepG2 cells. To determine whether ADK increases global DNA methylation and to validate its potential therapeutics, we treated HeLa cells with potent ADK inhibitors MRS4203 and MRS4380 (IC
Identifiants
pubmed: 33860193
doi: 10.1021/acsptsci.1c00008
pmc: PMC8033756
doi:
Types de publication
Journal Article
Langues
eng
Pagination
680-686Subventions
Organisme : NINDS NIH HHS
ID : R01 NS065957
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS103740
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA DK031117
Pays : United States
Informations de copyright
© 2021 American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
Références
J Med Chem. 1976 Sep;19(9):1094-9
pubmed: 978673
Methods Enzymol. 1990;193:782-90
pubmed: 1706062
Anal Biochem. 2011 Feb 1;409(1):138-43
pubmed: 20950581
Nat Genet. 1999 Feb;21(2):163-7
pubmed: 9988266
J Med Chem. 2016 Jul 28;59(14):6860-77
pubmed: 27410258
J Cell Sci. 2020 May 29;133(10):
pubmed: 32317394
Carcinogenesis. 2010 Jan;31(1):27-36
pubmed: 19752007
Nature. 1983 Jan 6;301(5895):89-92
pubmed: 6185846
Nat Rev Neurosci. 2016 Jul;17(7):411-23
pubmed: 27150399
Cancer Cell. 2019 Dec 9;36(6):582-596
pubmed: 31821783
Nat Metab. 2020 Apr;2(4):364-379
pubmed: 32694608
Acta Neuropathol. 2013 Nov;126(5):741-56
pubmed: 24005891
Oncogene. 2015 Jan 8;34(2):135-43
pubmed: 24469033
Front Immunol. 2019 Jun 06;10:925
pubmed: 31244820
Nat Rev Cancer. 2017 Nov 22;17(12):765
pubmed: 29162946
Pharmacol Rev. 2013 Apr 16;65(3):906-43
pubmed: 23592612
Anal Chem. 2005 Jan 15;77(2):504-10
pubmed: 15649046
Anal Biochem. 2009 Aug 15;391(2):106-13
pubmed: 19442645
Nature. 2012 Feb 15;483(7390):479-83
pubmed: 22343889
Nat Rev Drug Discov. 2006 Jan;5(1):37-50
pubmed: 16485345
Expert Rev Anticancer Ther. 2017 Jun;17(6):527-535
pubmed: 28399672
J Biol Chem. 1980 Nov 25;255(22):10822-7
pubmed: 7430157