Establishment of caprine airway epithelial cells grown in an air-liquid interface system to study caprine respiratory viruses and bacteria.

Animals Bronchi / cytology Cell Culture Techniques / methods Cell Differentiation Cells, Cultured Epithelial Cells / microbiology Goats Host-Pathogen Interactions Microscopy, Electron, Scanning Mycoplasma / physiology Respiratory Mucosa / microbiology Respiratory System / cytology Thogotovirus / physiology Viral Tropism Virus Replication / physiology
AECs ALI Air liquid interface Airway epithelial cell culture Goat Influenza D virus Mycoplasma mycoides subsp. capri

Journal

Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469

Informations de publication

Date de publication:
Jun 2021
Historique:
received: 08 02 2021
accepted: 06 04 2021
pubmed: 17 4 2021
medline: 25 9 2021
entrez: 16 4 2021
Statut: ppublish

Résumé

Respiratory diseases negatively impact the global goat industry, but are understudied. There is a shortage of established and biological relevant in vitro or ex vivo assays to study caprine respiratory infections. Here, we describe the establishment of an in vitro system based on well-differentiated caprine airway epithelial cell (AEC) cultures grown under air liquid interface conditions as an experimental platform to study caprine respiratory pathogens. The functional differentiation of the AEC cultures was monitored and confirmed by light and immunofluorescence microscopy, scanning electron microscopy and examination of histological sections. We validated the functionality of the platform by studying Influenza D Virus (IDV) infection and Mycoplasma mycoides subsp. capri (Mmc) colonization over 5 days, including monitoring of infectious agents by titration and qPCR as well as colour changing units, respectively. The inoculation of caprine AEC cultures with IDV showed that efficient viral replication takes place, and revealed that IDV has a marked cell tropism for ciliated cells. Furthermore, AEC cultures were successfully infected with Mmc using a multiplicity of infection of 0.1 and colonization was monitored over several days. Altogether, these results demonstrate that our newly-established caprine AEC cultures can be used to investigate host-pathogen interactions of caprine respiratory pathogens.

Identifiants

DOI: 10.1016/j.vetmic.2021.109067 PMID: 33862331
pubmed: 33862331
pii: S0378-1135(21)00090-0
doi: 10.1016/j.vetmic.2021.109067
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109067

Informations de copyright

Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Auteurs

Marina Strässle (M)

Institute of Virology and Immunology (IVI), Bern, Switzerland; Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Laura Laloli (L)

Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland; Institute for Infectious Diseases, University of Bern, Bern, Switzerland.

Mitra Gultom (M)

Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Graduate School for Biomedical Science, University of Bern, Bern, Switzerland; Institute for Infectious Diseases, University of Bern, Bern, Switzerland.

Philip V'kovski (P)

Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Michael H Stoffel (MH)

Division of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, Länggass-Str. 120, PO Box 3001, Bern, Switzerland.

Silvia Crespo Pomar (S)

Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland.

Astrid Chanfon Bätzner (A)

Institute of Animal Pathology (COMPATH), Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland.

Nadine Ebert (N)

Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

Fabien Labroussaa (F)

Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland.

Ronald Dijkman (R)

Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Institute for Infectious Diseases, University of Bern, Bern, Switzerland.

Joerg Jores (J)

Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggass-Str. 122, PO Box 3001, Bern, Switzerland. Electronic address: joerg.jores@vetsuisse.unibe.ch.

Volker Thiel (V)

Institute of Virology and Immunology (IVI), Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Establishment of caprine airway epithelial...
questionsmedicales.fr › Appareil respiratoire › Poumon › Bronches › Establishment of caprine airway epithelial...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Establishment of caprine airway epithelial...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Establishment of caprine airway epithelial...
questionsmedicales.fr › Cellules › Cellules cultivées › Establishment of caprine airway epithelial...
questionsmedicales.fr › Cellules › Cellules épithéliales › Establishment of caprine airway epithelial...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Capra › Establishment of caprine airway epithelial...
questionsmedicales.fr › Phénomènes biologiques › Interactions hôte-microbes › Interactions hôte-pathogène › Establishment of caprine airway epithelial...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à balayage › Establishment of caprine airway epithelial...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Tenericutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Establishment of caprine airway epithelial...
questionsmedicales.fr › Tissus › Membranes › Muqueuse › Muqueuse respiratoire › Establishment of caprine airway epithelial...
questionsmedicales.fr › Appareil respiratoire › Establishment of caprine airway epithelial...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Orthomyxoviridae › Thogotovirus › Establishment of caprine airway epithelial...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Tropisme viral › Establishment of caprine airway epithelial...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Establishment of caprine airway epithelial...