Innate immune response in bovine neutrophils stimulated with Mycoplasma bovis.

Animals Cattle Cattle Diseases / genetics Extracellular Traps / metabolism Gene Expression / immunology Immunity, Innate Mycoplasma Infections / genetics Mycoplasma bovis / physiology Neutrophils / immunology
Bovine Innate immune Mycoplasma bovis Neutrophils Transcriptome analysis

Journal

Veterinary research
ISSN: 1297-9716
Titre abrégé: Vet Res
Pays: England
ID NLM: 9309551

Informations de publication

Date de publication:
16 Apr 2021
Historique:
received: 04 08 2020
accepted: 28 02 2021
entrez: 17 4 2021
pubmed: 18 4 2021
medline: 24 8 2021
Statut: epublish

Résumé

Mycoplasma bovis (M. bovis) is a significant worldwide pathogen of cattle. Neutrophils have an important role in the innate immune response during infection with M. bovis. However, even though neutrophils accumulate in M. bovis infection, the interaction of M. bovis and neutrophils has not been fully elucidated. We attempted to elucidate the innate immune response of neutrophils stimulated with M. bovis and evaluate the transcriptome and functional analysis of bovine neutrophils stimulated with M. bovis. Proinflammatory cytokines, such as inducible nitric oxide (iNOS), which was the most increased gene in transcriptome analysis, were increased in quantitative polymerase chain reaction analysis of bovine neutrophils stimulated with live or heat-killed M. bovis. Nitric oxide and intracellular reactive oxygen species production of neutrophils stimulated with M. bovis was significantly increased. Neutrophils stimulated with M. bovis showed an increased ratio of nonapoptotic cell death compared to unstimulated controls. We demonstrated that neutrophil extracellular traps (NETs) formation was not recognized in neutrophils stimulated with live M. bovis. However, heat-killed M. bovis induced NETs formation. We also showed the interaction with M. bovis and bovine neutrophils regarding proinflammatory cytokine gene expression and functional expression related to NETs formation. Live and killed M. bovis induced innate immune responses in neutrophils and had the potential to induce NETs formation, but live M. bovis escaped NETs.

Identifiants

DOI: 10.1186/s13567-021-00920-2 PMID: 33863386 PMC: PMC8052696
pubmed: 33863386
doi: 10.1186/s13567-021-00920-2
pii: 10.1186/s13567-021-00920-2
pmc: PMC8052696
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

58

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Auteurs

Satoshi Gondaira (S)

Animal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.

Koji Nishi (K)

Animal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.

Jumpei Fujiki (J)

Veterinary Biochemistry, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.

Hidetomo Iwano (H)

Veterinary Biochemistry, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.

Reina Watanabe (R)

Animal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.

Ayako Eguchi (A)

Animal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.

Yuki Hirano (Y)

Animal Research Center, Agricultural Research Department, Hokkaido Research Organization, Shintoku, Hokkaido, 081-0038, Japan.

Hidetoshi Higuchi (H)

Animal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan. higuchi@rakuno.ac.jp.
ORCID: 0000-0002-7476-8544

Hajime Nagahata (H)

Animal Health Laboratory, Department of Veterinary Medicine, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.
Farm Animal Veterinary Nursing Laboratory, Department of Veterinary Associated Science, Faculty of Veterinary Medicine, Okayama University, Imabari, Ehime, 794-8555, Japan.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Innate immune response in bovine neutrophils...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › Innate immune response in bovine neutrophils...
questionsmedicales.fr › Maladies de l'animal › Maladies des bovins › Innate immune response in bovine neutrophils...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Pièges extracellulaires › Innate immune response in bovine neutrophils...
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Innate immune response in bovine neutrophils...
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité innée › Innate immune response in bovine neutrophils...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Mycoplasmatales › Infections à Mycoplasma › Innate immune response in bovine neutrophils...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Tenericutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma bovis › Innate immune response in bovine neutrophils...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Granulocytes neutrophiles › Innate immune response in bovine neutrophils...