Dietary influences on the Dahl SS rat gut microbiota and its effects on salt-sensitive hypertension and renal damage.
diet
faecal microbiota transfer
gut microbiota
hypertension
immune cells
Journal
Acta physiologica (Oxford, England)
ISSN: 1748-1716
Titre abrégé: Acta Physiol (Oxf)
Pays: England
ID NLM: 101262545
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
revised:
07
04
2021
received:
10
12
2020
accepted:
11
04
2021
pubmed:
19
4
2021
medline:
24
8
2021
entrez:
18
4
2021
Statut:
ppublish
Résumé
Our previous studies have demonstrated the importance of dietary factors in the determination of hypertension in Dahl salt-sensitive (SS) rats. Since the gut microbiota has been implicated in chronic diseases like hypertension, we hypothesized that dietary alterations shift the microbiota to mediate the development of salt-sensitive hypertension and renal disease. This study utilized SS rats from the Medical College of Wisconsin (SS/MCW) maintained on a purified, casein-based diet (0.4% NaCl AIN-76A, Dyets) and from Charles River Laboratories (SS/CRL) fed a whole grain diet (0.75% NaCl 5L79, LabDiet). Faecal 16S rDNA sequencing was used to phenotype the gut microbiota. Directly examining the contribution of the gut microbiota, SS/CRL rats were administered faecal microbiota transfer (FMT) experiments with either SS/MCW stool or vehicle (Vehl) in conjunction with the HS AIN-76A diet. SS/MCW rats exhibit renal damage and inflammation when fed high salt (HS, 4.0% NaCl AIN-76A), which is significantly attenuated in SS/CRL. Gut microbiota phenotyping revealed distinct profiles that correlate with disease severity. SS/MCW FMT worsened the SS/CRL response to HS, evidenced by increased albuminuria (67.4 ± 6.9 vs 113.7 ± 25.0 mg/day, Vehl vs FMT, P = .007), systolic arterial pressure (158.6 ± 5.8 vs 177.8 ± 8.9 mmHg, Vehl vs FMT, P = .09) and renal T-cell infiltration (1.9-fold). Amplicon sequence variant (ASV)-based analysis of faecal 16S rDNA sequencing data revealed taxa that significantly shifted with FMT: Erysipelotrichaceae_2, Parabacteroides gordonii, Streptococcus alactolyticus, Bacteroidales_1, Desulfovibrionaceae_2, Ruminococcus albus. These data demonstrate that dietary modulation of the gut microbiota directly contributes to the development of Dahl SS hypertension and renal injury.
Identifiants
pubmed: 33866692
doi: 10.1111/apha.13662
pmc: PMC9835005
mid: NIHMS1855981
doi:
Substances chimiques
Sodium Chloride, Dietary
0
Sodium Chloride
451W47IQ8X
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13662Subventions
Organisme : NHLBI NIH HHS
ID : HL137748
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL116264
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL137748
Pays : United States
Organisme : NHLBI NIH HHS
ID : F31 HL152495
Pays : United States
Organisme : NHLBI NIH HHS
ID : HL116264
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.
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