Direct measurement of pregnanediol 3-glucuronide (PDG) in dried urine spots by liquid chromatography-mass spectrometry to detect ovulation.
Female
Fertility
Human
Ovulation
Pregnanediol glucuronide
Urine
Journal
The Journal of steroid biochemistry and molecular biology
ISSN: 1879-1220
Titre abrégé: J Steroid Biochem Mol Biol
Pays: England
ID NLM: 9015483
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
18
02
2021
revised:
17
03
2021
accepted:
07
04
2021
pubmed:
20
4
2021
medline:
31
8
2021
entrez:
19
4
2021
Statut:
ppublish
Résumé
Non-invasive self-testing using an objective chemical method to detect ovulation is valuable for women planning conception, practising contraception or undergoing infertility investigations or treatment. Based on luteal phase secretion of progesterone (P Urine PDG with and without creatinine adjustment was stable during the follicular phase with the expected striking rise in the luteal phase peaking at 5 days after ovulation. Using a single spot urine sample (100 μL) or a DUS (<20 μL urine) and an optimal threshold to distinguish pre- from post-ovulatory samples, in ROC analysis urine PDG adjusted for creatinine accurately identified ovulation in 92 % of samples was comparable with P3G immunoassay and superior to urine P This method holds promise as a non-invasive self-test method for women to determine by an objective chemical method their ovulatory status.
Sections du résumé
BACKGROUND
Non-invasive self-testing using an objective chemical method to detect ovulation is valuable for women planning conception, practising contraception or undergoing infertility investigations or treatment.
METHODS
Based on luteal phase secretion of progesterone (P
RESULTS
Urine PDG with and without creatinine adjustment was stable during the follicular phase with the expected striking rise in the luteal phase peaking at 5 days after ovulation. Using a single spot urine sample (100 μL) or a DUS (<20 μL urine) and an optimal threshold to distinguish pre- from post-ovulatory samples, in ROC analysis urine PDG adjusted for creatinine accurately identified ovulation in 92 % of samples was comparable with P3G immunoassay and superior to urine P
CONCLUSIONS
This method holds promise as a non-invasive self-test method for women to determine by an objective chemical method their ovulatory status.
Identifiants
pubmed: 33872762
pii: S0960-0760(21)00093-5
doi: 10.1016/j.jsbmb.2021.105900
pii:
doi:
Substances chimiques
Biomarkers
0
pregnanediol-3 alpha-glucuronide
1852-49-9
Pregnanediol
JR3JD1Y22C
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105900Subventions
Organisme : Medical Research Council
ID : MR/N022556/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : MR/N022556/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 102419/Z/13/A
Pays : United Kingdom
Informations de copyright
Crown Copyright © 2021. Published by Elsevier Ltd. All rights reserved.