Spatial tissue profiling by imaging-free molecular tomography.
Journal
Nature biotechnology
ISSN: 1546-1696
Titre abrégé: Nat Biotechnol
Pays: United States
ID NLM: 9604648
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
03
08
2020
accepted:
05
03
2021
pubmed:
21
4
2021
medline:
22
9
2021
entrez:
20
4
2021
Statut:
ppublish
Résumé
Several techniques are currently being developed for spatially resolved omics profiling, but each new method requires the setup of specific detection strategies or specialized instrumentation. Here we describe an imaging-free framework to localize high-throughput readouts within a tissue by cutting the sample into thin strips in a way that allows subsequent image reconstruction. We implemented this framework to transform a low-input RNA sequencing protocol into an imaging-free spatial transcriptomics technique (called STRP-seq) and validated it by profiling the spatial transcriptome of the mouse brain. We applied the technique to the brain of the Australian bearded dragon, Pogona vitticeps. Our results reveal the molecular anatomy of the telencephalon of this lizard, providing evidence for a marked regionalization of the reptilian pallium and subpallium. We expect that STRP-seq can be used to derive spatially resolved data from a range of other omics techniques.
Identifiants
pubmed: 33875865
doi: 10.1038/s41587-021-00879-7
pii: 10.1038/s41587-021-00879-7
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
968-977Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.
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