Bile Microbiota in Patients with Pigment Common Bile Duct Stones.
Adult
Aged
Aged, 80 and over
Cholangiopancreatography, Endoscopic Retrograde
Common Bile Duct
/ microbiology
Discriminant Analysis
Enterococcus
/ genetics
Female
Firmicutes
/ genetics
Gallstones
/ diagnosis
Humans
Male
Microbiota
Middle Aged
Proteobacteria
/ genetics
RNA, Ribosomal, 16S
/ analysis
Young Adult
Choledocholithiasis
Enterococcus
Microbiota
Journal
Journal of Korean medical science
ISSN: 1598-6357
Titre abrégé: J Korean Med Sci
Pays: Korea (South)
ID NLM: 8703518
Informations de publication
Date de publication:
19 Apr 2021
19 Apr 2021
Historique:
received:
05
11
2020
accepted:
25
01
2021
entrez:
20
4
2021
pubmed:
21
4
2021
medline:
4
11
2021
Statut:
epublish
Résumé
Common bile duct (CBD) stone is one of the most prevalent gastroenterological diseases, but the role played by biliary microbiota in the pathogenesis of CBD stones remains obscure. The aim of this study was to investigate the characteristics of the biliary tract core microbiome and its potential association with the formation of pigment stones. Twenty-eight patients with biliary obstruction of various causes were enrolled. Thirteen had new-onset pigment CBD stone. Of the remaining 15, four had benign biliary stricture, four had gallbladder cancer, three had pancreatic cancer, 3 had distal CBD cancer, and one had hepatocellular carcinoma. Endoscopic retrograde cholangiopancreatography was used to collect bile samples for DNA extraction, 16S ribosomal RNA gene sequencing, and bile microbiota composition analysis. Proteobacteria (61.7%), Firmicutes (25.1%), Bacteroidetes (5%), Fusobacteria (4.6%), and Actinobacteria (2.6%) were the most dominant phyla in the bile of the 28 study subjects. A comparison between new-onset choledocholithiasis and other causes of biliary obstruction (controls) showed This study demonstrates an abundance of microbiota in bile juice and presents a biliary microbiome composition similar to that of duodenum. The study also shows
Sections du résumé
BACKGROUND
BACKGROUND
Common bile duct (CBD) stone is one of the most prevalent gastroenterological diseases, but the role played by biliary microbiota in the pathogenesis of CBD stones remains obscure. The aim of this study was to investigate the characteristics of the biliary tract core microbiome and its potential association with the formation of pigment stones.
METHODS
METHODS
Twenty-eight patients with biliary obstruction of various causes were enrolled. Thirteen had new-onset pigment CBD stone. Of the remaining 15, four had benign biliary stricture, four had gallbladder cancer, three had pancreatic cancer, 3 had distal CBD cancer, and one had hepatocellular carcinoma. Endoscopic retrograde cholangiopancreatography was used to collect bile samples for DNA extraction, 16S ribosomal RNA gene sequencing, and bile microbiota composition analysis.
RESULTS
RESULTS
Proteobacteria (61.7%), Firmicutes (25.1%), Bacteroidetes (5%), Fusobacteria (4.6%), and Actinobacteria (2.6%) were the most dominant phyla in the bile of the 28 study subjects. A comparison between new-onset choledocholithiasis and other causes of biliary obstruction (controls) showed
CONCLUSION
CONCLUSIONS
This study demonstrates an abundance of microbiota in bile juice and presents a biliary microbiome composition similar to that of duodenum. The study also shows
Identifiants
pubmed: 33876584
pii: 36.e94
doi: 10.3346/jkms.2021.36.e94
pmc: PMC8055513
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e94Subventions
Organisme : Korean Society of Gastroenterology
Pays : Korea
Organisme : National Research Foundation of Korea
ID : NRF-2019M3E5D1A02069613
Pays : Korea
Informations de copyright
© 2021 The Korean Academy of Medical Sciences.
Déclaration de conflit d'intérêts
The authors have no potential conflicts of interest to disclose.
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