Structural basis for GTP-induced dimerization and antiviral function of guanylate-binding proteins.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
13 04 2021
Historique:
entrez: 20 4 2021
pubmed: 21 4 2021
medline: 27 11 2021
Statut: ppublish

Résumé

Guanylate-binding proteins (GBPs) form a family of dynamin-related large GTPases which mediate important innate immune functions. They were proposed to form oligomers upon GTP binding/hydrolysis, but the molecular mechanisms remain elusive. Here, we present crystal structures of C-terminally truncated human GBP5 (hGBP5

Identifiants

pubmed: 33876762
pii: 2022269118
doi: 10.1073/pnas.2022269118
pmc: PMC8054025
pii:
doi:

Substances chimiques

GBP5 protein, human 0
env Gene Products, Human Immunodeficiency Virus 0
Guanosine Triphosphate 86-01-1
GBP2 protein, human EC 3.6.1.-
GTP-Binding Proteins EC 3.6.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

Wen Cui (W)

School of Life Sciences, Tianjin University, Tianjin, 300072, China.
Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Institute of Life Sciences, Chongqing Medical University, Chongqing, 400016, China.

Elisabeth Braun (E)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.

Wei Wang (W)

Institute of Life Sciences, Chongqing Medical University, Chongqing, 400016, China.

Jinhong Tang (J)

School of Life Sciences, Tianjin University, Tianjin, 300072, China.

Yanyan Zheng (Y)

Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 510006 Guangzhou, China.

Benjamin Slater (B)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520.

Na Li (N)

National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, Shanghai, 201210, China.

Cheng Chen (C)

School of Life Sciences, Tianjin University, Tianjin, 300072, China.

Qingxiang Liu (Q)

Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 510006 Guangzhou, China.

Bin Wang (B)

School of Life Sciences, Tianjin University, Tianjin, 300072, China.

Xiu Li (X)

School of Life Sciences, Tianjin University, Tianjin, 300072, China.

Yinkai Duan (Y)

Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.

Yunjie Xiao (Y)

School of Life Sciences, Tianjin University, Tianjin, 300072, China.

Ruijiao Ti (R)

School of Life Sciences, Tianjin University, Tianjin, 300072, China.

Dominik Hotter (D)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.

Xiaoyun Ji (X)

Institute of Life Sciences, Chongqing Medical University, Chongqing, 400016, China.
State Key Laboratory of Pharmaceutical Biotechnology, Department of Biotechnology and Pharmaceutical Sciences, School of Life Sciences, Nanjing University, Nanjing, 210023, China.

Lei Zhang (L)

School of Life Sciences, Tianjin University, Tianjin, 300072, China.

Jun Cui (J)

Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 510006 Guangzhou, China.

Yong Xiong (Y)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520.

Daniel Sauter (D)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; Daniel.Sauter@med.uni-tuebingen.de zefangwang@tju.edu.cn frank.kirchhoff@uni-ulm.de yanght@shanghaitech.edu.cn.
Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Tübingen, 72076, Germany.

Zefang Wang (Z)

School of Life Sciences, Tianjin University, Tianjin, 300072, China; Daniel.Sauter@med.uni-tuebingen.de zefangwang@tju.edu.cn frank.kirchhoff@uni-ulm.de yanght@shanghaitech.edu.cn.
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China.

Frank Kirchhoff (F)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany; Daniel.Sauter@med.uni-tuebingen.de zefangwang@tju.edu.cn frank.kirchhoff@uni-ulm.de yanght@shanghaitech.edu.cn.

Haitao Yang (H)

School of Life Sciences, Tianjin University, Tianjin, 300072, China; Daniel.Sauter@med.uni-tuebingen.de zefangwang@tju.edu.cn frank.kirchhoff@uni-ulm.de yanght@shanghaitech.edu.cn.
Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, 300457, China.

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Classifications MeSH