Programming Fluorogenic DNA Probes for Rapid Detection of Steroids.
DNA
aptamers
detection
forced intercalation
steroids
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
05 07 2021
05 07 2021
Historique:
revised:
13
04
2021
received:
09
03
2021
pubmed:
21
4
2021
medline:
16
9
2021
entrez:
20
4
2021
Statut:
ppublish
Résumé
The ability of aptamers to recognize a variety of different molecules has fueled their emergence as recognition agents to probe complex media and cells. Many detection strategies require aptamer binding to its target to result in a dramatic change in structure, typically from an unfolded to a folded state. Here, we report a strategy based on forced intercalation (FIT) that increases the scope of aptamer recognition by transducing subtle changes in aptamer structures into fluorescent readouts. By screening a library of green-fluorescent FIT-aptamers whose design is guided by computational modeling, we could identify hits that sense steroids like dehydroepiandrosterone sulfate (DHEAS) down to 1.3 μM with no loss in binding affinity compared to the unmodified aptamer. This enabled us to study DHEAS in clinical serum samples with several advantages over gold standard methods, including rapid readout (<30 min), simple instrumentation (plate-reader), and low sample volumes (10 μL).
Identifiants
pubmed: 33878237
doi: 10.1002/anie.202103440
pmc: PMC8396052
mid: NIHMS1733956
doi:
Substances chimiques
DNA Probes
0
Fluorescent Dyes
0
Steroids
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
15260-15265Subventions
Organisme : NCI NIH HHS
ID : P30 CA060553
Pays : United States
Organisme : NCI NIH HHS
ID : CCSG P30 CA060553
Pays : United States
Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
Nat Protoc. 2006;1(3):1057-65
pubmed: 17406387
J Am Chem Soc. 2007 Dec 19;129(50):15477-9
pubmed: 18034495
J Am Chem Soc. 2019 Sep 4;141(35):13744-13748
pubmed: 31441661
J Am Chem Soc. 2020 Jul 1;142(26):11343-11356
pubmed: 32573219
J Mol Endocrinol. 2016 Apr;56(3):R139-55
pubmed: 26908835
Chem Rev. 2008 Feb;108(2):423-61
pubmed: 18229952
Adv Mater. 2020 Apr;32(13):e1901743
pubmed: 31271253
J Clin Endocrinol Metab. 1972 Jun;34(6):1039-54
pubmed: 4336456
J Am Chem Soc. 2020 Aug 5;142(31):13350-13355
pubmed: 32706250
Nat Biotechnol. 1996 Mar;14(3):303-8
pubmed: 9630890
Nat Chem. 2020 Feb;12(2):165-172
pubmed: 31792385
Chem Sci. 2016 Jan 1;7(1):128-135
pubmed: 29861973
Nat Rev Endocrinol. 2020 May;16(5):284-296
pubmed: 32203405
Nature. 1996 Aug 15;382(6592):607-9
pubmed: 8757129
Science. 2018 Oct 19;362(6412):319-324
pubmed: 30190311
Chem Rev. 2010 May 12;110(5):2620-40
pubmed: 20000749
J Biotechnol. 2000 Mar;74(1):5-13
pubmed: 10943568
J Am Chem Soc. 2012 Jan 25;134(3):1642-7
pubmed: 22142383
Mikrochim Acta. 2019 Jul 24;186(8):563
pubmed: 31338623
Angew Chem Int Ed Engl. 2020 Aug 10;59(33):13734-13762
pubmed: 31605413
N Engl J Med. 2005 Oct 20;353(16):1711-23
pubmed: 16236742
Science. 1990 Aug 3;249(4968):505-10
pubmed: 2200121
Diagnostics (Basel). 2020 Aug 14;10(8):
pubmed: 32823852
Chembiochem. 2005 Jan;6(1):69-77
pubmed: 15584015
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15
pubmed: 12824337
Chem Rev. 2001 Feb;101(2):445-77
pubmed: 11712254
J Am Chem Soc. 2003 Apr 23;125(16):4771-8
pubmed: 12696895
Nat Nanotechnol. 2009 May;4(5):325-30
pubmed: 19421220
Nature. 1990 Aug 30;346(6287):818-22
pubmed: 1697402
Science. 2012 Mar 9;335(6073):1194
pubmed: 22403384
Steroids. 2007 Jan;72(1):71-84
pubmed: 17157339
Acc Chem Res. 2016 May 17;49(5):775-83
pubmed: 26990857
Anal Bioanal Chem. 2008 Feb;390(4):1093-9
pubmed: 18165929
Drug Metab Rev. 2006;38(1-2):89-116
pubmed: 16684650
Chem Rev. 2014 Aug 13;114(15):7631-77
pubmed: 25115973
ACS Sens. 2018 Oct 26;3(10):2025-2044
pubmed: 30264989
Nano Lett. 2009 Sep;9(9):3258-61
pubmed: 19645478
Nat Rev Endocrinol. 2013 Nov;9(11):670-86
pubmed: 24080732
Nat Rev Drug Discov. 2017 Mar;16(3):181-202
pubmed: 27807347