Arterial Platelet Adhesion in Atherosclerosis-Prone Arteries of Obese, Insulin-Resistant Nonhuman Primates.
Animals
Atherosclerosis
/ metabolism
Biomarkers
/ metabolism
Blood Platelets
/ metabolism
Carotid Arteries
/ metabolism
Carotid Intima-Media Thickness
Disease Models, Animal
Endothelium, Vascular
/ metabolism
Insulin
/ metabolism
Insulin Resistance
/ physiology
Macaca mulatta
Male
Molecular Imaging
/ methods
Obesity
/ metabolism
Platelet Adhesiveness
/ physiology
Vascular Stiffness
/ physiology
atherosclerosis
molecular imaging
platelets
von Willebrand factor
Journal
Journal of the American Heart Association
ISSN: 2047-9980
Titre abrégé: J Am Heart Assoc
Pays: England
ID NLM: 101580524
Informations de publication
Date de publication:
04 05 2021
04 05 2021
Historique:
pubmed:
22
4
2021
medline:
26
10
2021
entrez:
21
4
2021
Statut:
ppublish
Résumé
Background Platelet-endothelial interactions are thought to contribute to early atherogenesis. These interactions are potentiated by oxidative stress. We used in vivo molecular imaging to test the hypothesis that platelet-endothelial interactions occur at early stages of plaque development in obese, insulin-resistant nonhuman primates, and are suppressed by NADPH-oxidase-2 inhibition. Methods and Results Six adult rhesus macaques fed a Western-style diet for a median of 4.0 years were studied at baseline and after 8 weeks of therapy with the NADPH-oxidase-2-inhibitor apocynin (50 mg/kg per day). Six lean control animals were also studied. Measurements included intravenous glucose tolerance test, body composition by dual-energy X-ray absorptiometry, carotid intimal medial thickness, carotid artery contrast ultrasound molecular imaging for platelet GPIbα (glycoprotein- Ibα) and vascular cell adhesion molecule-1, and blood oxidative markers on mass spectrometry. Compared with lean controls, animals on a Western-style diet were obese (median body mass: 16.0 versus 8.7 kg,
Identifiants
pubmed: 33880941
doi: 10.1161/JAHA.120.019413
pmc: PMC8200741
doi:
Substances chimiques
Biomarkers
0
Insulin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e019413Subventions
Organisme : NIH HHS
ID : P51 OD011092
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL078610
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL130046
Pays : United States
Organisme : NHLBI NIH HHS
ID : R35 HL145262
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL137991
Pays : United States
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