Screening and Purification of Natural Products from Actinomycetes that Induce a "Rounded" Morphological Phenotype in Fission Yeast.
Actinomycetes
Antifungal
Morphology
Phenotype
Polyene
Schizosaccharomyces pombe
Journal
Natural products and bioprospecting
ISSN: 2192-2195
Titre abrégé: Nat Prod Bioprospect
Pays: Germany
ID NLM: 101577734
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
28
01
2021
accepted:
06
04
2021
pubmed:
22
4
2021
medline:
22
4
2021
entrez:
21
4
2021
Statut:
ppublish
Résumé
This study was designed to identify and investigate bioactive natural product compounds that alter the cellular shape of the fission yeast Schizosaccharomyces pombe and induce a "rounded" or "small" cellular morphological phenotype. Bioassays using a range of antifungal agents against a multidrug-sensitive fission yeast strain, SAK950 showed that many induced a "rounded" phenotype. We then investigated whether 46 of the actinomycete strains identified in our previous study as inducing a similar phenotype produced antifungal agents of similar classes. We show that five of the strains produced streptothricin and that 26 strains produced polyenes, including fungichromin, filipin and candicidin, the last of which was produced by 24 strains. A taxonomic study of the strains indicated that the majority of the candicidin only producers were Streptomyces hydrogenans and S. albidoflavus whilst those that additionally produced streptothricin were related to S. enissocaesilis. A follow-up study to investigate the natural products made by related strains indicated that they followed a similar pattern. The identification of several compounds from the actinomycete strains similar to the antifungal agents initially tested confirm the validity of an approach using the S. pombe morphological phenotype and actinomycete taxonomy as a predictive tool for natural product identification.
Identifiants
pubmed: 33881756
doi: 10.1007/s13659-021-00304-1
pii: 10.1007/s13659-021-00304-1
pmc: PMC8275771
doi:
Types de publication
Journal Article
Langues
eng
Pagination
431-445Subventions
Organisme : Wellcome Trust
ID : 093917
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : FC001121
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Cancer Research UK
ID : FC001121
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001121
Pays : United Kingdom
Informations de copyright
© 2021. The Author(s).
Références
Cell Cycle. 2014;13(8):1327-34
pubmed: 24621506
J Cell Biol. 2015 Sep 14;210(6):871-82
pubmed: 26370497
J Antibiot (Tokyo). 2008 Nov;61(11):660-7
pubmed: 19168980
Sci Rep. 2018 May 2;8(1):6888
pubmed: 29720592
J Cell Sci. 2017 Sep 15;130(18):3173-3185
pubmed: 28775153
PLoS One. 2015 Jan 30;10(1):e0116457
pubmed: 25635820
J Gen Microbiol. 1958 Jun;18(3):649-57
pubmed: 13549696
Metab Eng. 2011 Nov;13(6):756-67
pubmed: 22001323
Antibiotiki. 1973 Apr;18(4):354-8
pubmed: 4767286
Int J Syst Evol Microbiol. 2017 May;67(5):1613-1617
pubmed: 28005526
J Gen Microbiol. 1957 Aug;17(1):96-103
pubmed: 13475677
Methods Enzymol. 1991;194:795-823
pubmed: 2005825
Clin Infect Dis. 2003 Jan 1;36(1):70-8
pubmed: 12491205
J Hosp Infect. 2002 Aug;51(4):288-96
pubmed: 12183144
Chem Biol. 2008 Jun;15(6):629-38
pubmed: 18559273
Microb Cell Fact. 2015 Aug 07;14:114
pubmed: 26246267
Nat Chem Biol. 2014 Nov;10(11):963-8
pubmed: 25262415
Antibiotiki. 1969 Jun;14(6):505-8
pubmed: 4979791
Antibiot Annu. 1955-1956;3:579-86
pubmed: 13355330
J Mol Evol. 1981;17(6):368-76
pubmed: 7288891
Bull World Health Organ. 1965;33(2):219-26
pubmed: 5320588
Clin Infect Dis. 2008 Feb 15;46(4):625-33
pubmed: 18190281
Proc Soc Exp Biol Med. 1951 Jan;76(1):93-7
pubmed: 14816400
J Med Microbiol. 2007 May;56(Pt 5):645-649
pubmed: 17446287
J Antibiot (Tokyo). 2015 May;68(5):328-33
pubmed: 25464973
J Antimicrob Chemother. 1998 Jan;41(1):127-30
pubmed: 9511049
Indian J Crit Care Med. 2015 Mar;19(3):151-4
pubmed: 25810610
Med Mycol. 2017 Jul 1;55(5):563-567
pubmed: 28204557
Biosci Biotechnol Biochem. 2013;77(5):1086-91
pubmed: 23649273
Clin Microbiol Rev. 2007 Jan;20(1):133-63
pubmed: 17223626
Sci Transl Med. 2012 Dec 19;4(165):165rv13
pubmed: 23253612
Appl Environ Microbiol. 2009 May;75(10):3296-303
pubmed: 19286787
Appl Microbiol Biotechnol. 2017 May;101(10):4247-4257
pubmed: 28238083
Front Microbiol. 2016 Jun 29;7:1004
pubmed: 27446043
Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):E1130-9
pubmed: 24616526
Can J Microbiol. 2003 Nov;49(11):669-74
pubmed: 14735216
J Cell Sci. 2005 Mar 1;118(Pt 5):1033-44
pubmed: 15731009
Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4742-6
pubmed: 19270078
Scand J Infect Dis Suppl. 1976;(9):62-6
pubmed: 795012
Clin Infect Dis. 2001 Dec 1;33(11):1824-33
pubmed: 11692293
Curr Biol. 2005 Jul 26;15(14):1331-6
pubmed: 16051179
Mol Biol Cell. 2011 Oct;22(20):3801-11
pubmed: 21849474
J Antibiot (Tokyo). 1972 Sep;25(9):501-8
pubmed: 4645946
J Cell Biol. 1995 Dec;131(6 Pt 1):1529-38
pubmed: 8522609
Microbiology (Reading). 2008 Mar;154(Pt 3):830-841
pubmed: 18310029
Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2234-9
pubmed: 22308411
J Fungi (Basel). 2016 Oct 02;2(4):
pubmed: 29376943
Microb Biotechnol. 2014 May;7(3):242-56
pubmed: 24593309
Nat Cell Biol. 2004 Nov;6(11):1142-4
pubmed: 15517003
Traffic. 2008 Jun;9(6):936-50
pubmed: 18346214
Nat Rev Drug Discov. 2017 Sep;16(9):603-616
pubmed: 28496146
Antonie Van Leeuwenhoek. 2013 May;103(5):1107-11
pubmed: 23444037
J Antibiot (Tokyo). 1989 Apr;42(4):577-84
pubmed: 2722673
J Basic Microbiol. 2012 Apr;52(2):150-9
pubmed: 22460913
J Biomol Screen. 2013 Dec;18(10):1143-55
pubmed: 24080259
PLoS One. 2011;6(8):e22028
pubmed: 21857911
FEMS Microbiol Lett. 1990 Jun 15;58(1):15-8
pubmed: 2397878
Microbiology (Reading). 2008 Mar;154(Pt 3):960-970
pubmed: 18310042
J Cell Sci. 2003 Mar 1;116(Pt 5):867-74
pubmed: 12571284
Evolution. 1985 Jul;39(4):783-791
pubmed: 28561359
Appl Microbiol Biotechnol. 2014 Nov;98(22):9311-24
pubmed: 25104037
BMC Genomics. 2014 Apr 30;15:323
pubmed: 24884595
Mol Biol Evol. 1993 May;10(3):512-26
pubmed: 8336541
J Cell Sci. 1985 Apr;75:357-76
pubmed: 4044680
Eur J Cell Biol. 2005 Dec;84(12):915-26
pubmed: 16325501
Mol Microbiol. 2008 May;68(4):813-26
pubmed: 18399939
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
J Ind Microbiol. 1994 May;13(3):183-9
pubmed: 7764844
J Bacteriol. 1991 Jun;173(11):3456-62
pubmed: 1828464
J Basic Microbiol. 2014 Nov;54(11):1175-85
pubmed: 23765423
Nat Prod Bioprospect. 2014 Feb;4(1):27-35
pubmed: 24660134
J Comput Biol. 2010 Mar;17(3):337-54
pubmed: 20377449
Appl Environ Microbiol. 2012 Jan;78(2):589-92
pubmed: 22057866
AIDS. 2009 Feb 20;23(4):525-30
pubmed: 19182676
Appl Microbiol Biotechnol. 2015 Jun;99(12):5123-35
pubmed: 25715784
Clin Microbiol Infect. 2010 Jul;16(7):870-7
pubmed: 19906275
BMC Genomics. 2014 Nov 15;15:970
pubmed: 25399205
Appl Environ Microbiol. 2013 Oct;79(19):6156-9
pubmed: 23913424