Single-Dilution COVID-19 Antibody Test with Qualitative and Quantitative Readouts.
Adolescent
Adult
Aged
Antibodies, Viral
/ blood
Antibody Specificity
COVID-19
/ diagnosis
COVID-19 Serological Testing
/ methods
Case-Control Studies
Cohort Studies
Enzyme-Linked Immunosorbent Assay
/ methods
Epidemiological Monitoring
Female
Humans
Immunoglobulin A
/ blood
Immunoglobulin G
/ blood
Male
Middle Aged
Pandemics
SARS-CoV-2
/ immunology
Seroepidemiologic Studies
Spike Glycoprotein, Coronavirus
/ immunology
Young Adult
COVID-19
IgA
IgG
SARS-CoV-2
laboratory diagnostic test
quantitative test
serology
spike protein
Journal
mSphere
ISSN: 2379-5042
Titre abrégé: mSphere
Pays: United States
ID NLM: 101674533
Informations de publication
Date de publication:
21 04 2021
21 04 2021
Historique:
entrez:
22
4
2021
pubmed:
23
4
2021
medline:
30
4
2021
Statut:
epublish
Résumé
The coronavirus disease 2019 (COVID-19) global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to place an immense burden on societies and health care systems. A key component of COVID-19 control efforts is serological testing to determine the community prevalence of SARS-CoV-2 exposure and quantify individual immune responses to prior SARS-CoV-2 infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents to detect SARS-CoV-2 exposure in patient blood samples with high sensitivity and specificity. We further show that this sensitive test affords the estimation of viral spike-specific IgG titers from a single sample measurement, thereby providing a simple and scalable method to measure the strength of an individual's immune response. The accuracy, adaptability, and cost-effectiveness of this test make it an excellent option for clinical deployment in the ongoing COVID-19 pandemic.
Identifiants
pubmed: 33883259
pii: 6/2/e00224-21
doi: 10.1128/mSphere.00224-21
pmc: PMC8546701
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin A
0
Immunoglobulin G
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : AI13261
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI124753
Pays : United States
Organisme : NCI NIH HHS
ID : K08 CA143153
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI134753
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA236546
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007288
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145024
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI132633
Pays : United States
Organisme : NIAID NIH HHS
ID : F30 AI150055
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI125462
Pays : United States
Organisme : NIGMS NIH HHS
ID : 2T32GM007288-45
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI124414
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002556
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2021 Bortz et al.
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