A comprehensive characterization of the cell-free transcriptome reveals tissue- and subtype-specific biomarkers for cancer detection.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
21 04 2021
21 04 2021
Historique:
received:
03
08
2020
accepted:
10
03
2021
entrez:
22
4
2021
pubmed:
23
4
2021
medline:
4
5
2021
Statut:
epublish
Résumé
Cell-free RNA (cfRNA) is a promising analyte for cancer detection. However, a comprehensive assessment of cfRNA in individuals with and without cancer has not been conducted. We perform the first transcriptome-wide characterization of cfRNA in cancer (stage III breast [n = 46], lung [n = 30]) and non-cancer (n = 89) participants from the Circulating Cell-free Genome Atlas (NCT02889978). Of 57,820 annotated genes, 39,564 (68%) are not detected in cfRNA from non-cancer individuals. Within these low-noise regions, we identify tissue- and cancer-specific genes, defined as "dark channel biomarker" (DCB) genes, that are recurrently detected in individuals with cancer. DCB levels in plasma correlate with tumor shedding rate and RNA expression in matched tissue, suggesting that DCBs with high expression in tumor tissue could enhance cancer detection in patients with low levels of circulating tumor DNA. Overall, cfRNA provides a unique opportunity to detect cancer, predict the tumor tissue of origin, and determine the cancer subtype.
Identifiants
pubmed: 33883548
doi: 10.1038/s41467-021-22444-1
pii: 10.1038/s41467-021-22444-1
pmc: PMC8060291
doi:
Substances chimiques
Biomarkers, Tumor
0
Cell-Free Nucleic Acids
0
RNA, Messenger
0
Banques de données
ClinicalTrials.gov
['NCT02889978']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
2357Commentaires et corrections
Type : ErratumIn
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