Evaluation of Acute and Sub-Acute Toxicity of Aqueous Extracts of
A. afra
Swiss albino mice
Toxicity study
brain
heart
histopathology
suprarenal glands
Journal
Ethiopian journal of health sciences
ISSN: 2413-7170
Titre abrégé: Ethiop J Health Sci
Pays: Ethiopia
ID NLM: 101224773
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
entrez:
22
4
2021
pubmed:
23
4
2021
medline:
19
8
2021
Statut:
ppublish
Résumé
The majority of population rely on traditional medicine as a source of healthcare. Artemisia afra is a plant traditionally used for its medicinal values, including treatment of malaria in many parts of the world. Currently, it is also attracting attention because of a claim that a related species, Artemisia annua, is a remedy for the COVD-19 pandemic. The aim of the present study was to investigate toxic effects of A. afra on brain, heart and suprarenal glands in mice aged 8-12 weeks and weighing 25-30g. Leaves of A.afra were collected from Bale National Park, dried under shade, crushed into powder and soaked in distilled water to yield aqueous extract for oral administration. For acute toxicity study, seven treated and one control groups, with 3 female mice each, were used. They were given a single dose of 200mg/kg, 700mg/kg, 1200mg/kg, 2200mg/kg, 3200mg/kg, 4200mg/kg or 5000mg/kg b/wt of the extract. For the sub-acute toxicity study, two treated and one control groups, with 5 female and 5 male mice each, were used. They were daily treated with 600mg/kg or 1800mg/kg b/wt of extract. LD The findings of this study suggest that the leaves extract of A. afra is relatively safe in mice.
Sections du résumé
BACKGROUND
BACKGROUND
The majority of population rely on traditional medicine as a source of healthcare. Artemisia afra is a plant traditionally used for its medicinal values, including treatment of malaria in many parts of the world. Currently, it is also attracting attention because of a claim that a related species, Artemisia annua, is a remedy for the COVD-19 pandemic. The aim of the present study was to investigate toxic effects of A. afra on brain, heart and suprarenal glands in mice aged 8-12 weeks and weighing 25-30g.
METHODS
METHODS
Leaves of A.afra were collected from Bale National Park, dried under shade, crushed into powder and soaked in distilled water to yield aqueous extract for oral administration. For acute toxicity study, seven treated and one control groups, with 3 female mice each, were used. They were given a single dose of 200mg/kg, 700mg/kg, 1200mg/kg, 2200mg/kg, 3200mg/kg, 4200mg/kg or 5000mg/kg b/wt of the extract. For the sub-acute toxicity study, two treated and one control groups, with 5 female and 5 male mice each, were used. They were daily treated with 600mg/kg or 1800mg/kg b/wt of extract.
RESULTS
RESULTS
LD
CONCLUSION
CONCLUSIONS
The findings of this study suggest that the leaves extract of A. afra is relatively safe in mice.
Identifiants
pubmed: 33883843
doi: 10.4314/ejhs.v30i6.16
pii: jEJHS.v30.i6.pg981
pmc: PMC8047245
doi:
Substances chimiques
Plant Extracts
0
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
981-990Informations de copyright
© 2020 Ketema Mekonnen., et al.
Références
Toxicol Res. 2011 Dec;27(4):261-7
pubmed: 24278581
J Ethnopharmacol. 2010 Sep 15;131(2):404-9
pubmed: 20643198
Toxicol Pathol. 2001 Jan-Feb;29(1):41-8
pubmed: 11215683
Regul Toxicol Pharmacol. 2013 Feb;65(1):100-7
pubmed: 23201408
Toxicol Pathol. 2004 Jul-Aug;32(4):448-66
pubmed: 15204968
Toxicology. 2002 Oct 15;179(3):183-96
pubmed: 12270592
Afr J Tradit Complement Altern Med. 2012 Oct 01;10(1):15-20
pubmed: 24082320
Biomed Res Int. 2013;2013:804086
pubmed: 24163821
Evid Based Complement Alternat Med. 2013;2013:929074
pubmed: 23861717
J Ethnopharmacol. 1994 Dec;44(3):199-209
pubmed: 7898127