Simultaneous separation and determination of four active ingredients in Picria fel-terrae Lour. and its preparations by micellar electrokinetic chromatography.
Picriae Herba
active ingredients
micellar electrokinetic chromatography
mixed organic modifiers
simultaneous determination
Journal
Phytochemical analysis : PCA
ISSN: 1099-1565
Titre abrégé: Phytochem Anal
Pays: England
ID NLM: 9200492
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
26
03
2021
received:
23
02
2021
accepted:
01
04
2021
pubmed:
23
4
2021
medline:
13
10
2021
entrez:
22
4
2021
Statut:
ppublish
Résumé
Picfeltarraenins IA, IB and IV and acteoside are the four bioactive ingredients of Picria fel-terrae Lour. Their pharmacological effects include central inhibitory, cardiovascular, anti-inflammatory, anti-pyretic, analgesic, anti-bacterial, antioxidative and anti-tumor effects. We aimed to develop an efficient micellar electrokinetic chromatography (MEKC) method modified with mixed organic solvents for the simultaneous separation and determination of the four components in Picriae Herba and its formulations. Method optimization was carried out by investigating influences of significant factors on the separation, and this method was successfully applied for the determination of the four components in Picriae Herba and its formulations. The optimal running buffer was composed of 20 mM sodium tetraborate, 40 mM sodium cholate, 10% (v/v) methanol and 10% (v/v) isopropanol (pH 9.76). The separation voltage was 18 kV, the temperature was 25°C and the detection wavelength was 266 nm. Under the optimal separation conditions, the baseline separation of four components was achieved in less than 14 min. The correlation coefficients of the calibration curves were 0.9984-0.9995 for the analytes. The intraday and interday precision ranged from 1.5% to 2.5% and from 1.4% to 5.0%, respectively. Recoveries of analytes varied from 96.6% to 104.1%. The method was proved suitable for the determination of four components in Picriae Herba and its formulations. Good performance was obtained under optimal conditions, and the method provides an effective tool for the quality control of Picriae Herba and its formulations.
Substances chimiques
Micelles
0
Solvents
0
Sodium Cholate
NU3Y4CCH8Z
Methanol
Y4S76JWI15
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1110-1117Subventions
Organisme : Scientific Research Project of Health Commission of Shanxi Province
ID : 2020-45
Organisme : the Natural Science Foundation of Shanxi Province
ID : 201701D121141
Organisme : the Natural Science Foundation of Shanxi Province
ID : 201801D221406
Informations de copyright
© 2021 John Wiley & Sons, Ltd.
Références
Wen L, Wei Q, Chen G, Liu F, Zhang S, You T. Bioassay- and liquid chromatography/mass spectrometry-guided acetylcholinesterase inhibitors from Picriafel-terrae. Pharmacogn Mag. 2013;9:25-31.
Kumarasingha R, Preston S, Yeo TC, et al. Anthelmintic activity of selected ethno-medicinal plant extracts on parasitic stages of Haemonchus contortus. Parasit Vectors. 2016;9:187-193.
Satria D, Silalahi J, Haro G, Ilyas S, Hsb PA. Antioxidant and antiproliferative activities of an ethylacetate fraction of Picria fel-terrae Lour, Herbs. Asian Pac J Cancer Prev. 2017;18(2):399-403.
Auliafendri N. Rosidah, Yuandani, Suryani S, Satria D. The immunomodulatory activities of Picria fel-terrae Lour herbs towards RAW 264.7 Cells. Open Access Maced J Med Sci. 2019;7(1):24-28.
Shi R, Wang Q, Ouyang Y, Wang Q, Xiong X. Picfeltarraenin IA inhibits lipopolysaccharide-induced inflammatory cytokine production by the nuclear factor-kappa B pathway in human pulmonary epithelial A549 cells. Oncol Lett. 2016;11(2):1195-1200.
Lestari P, Hadisahputra S, Ilyas S, Satria D. Combinational effects of n-hexane extract of poguntano leaves (Picria fel-terrae Lour.) with doxorubicin on MCF-7 breast cancer cells. J Chem Pharm Res. 2015;7(5):353-355.
Huang Y, Apers S, De Bruyne T, et al. Flavonoid glucuronides from Picria fel-terrae. Phytochemistry. 1999;52(8):1701-1703.
Zou JM, Wang LS, Niu XM, Sun HD, Guo YJ. Phenylethanoid glycosides from Picria felterrae Lour. J Bot. 2005;47(5):632-636.
Huang Y, De Bruyne T, Apers S, et al. Complement-inhibiting cucurbitacin glycosides from Picria fel-terrae. J Nat Prod. 1998;61(6):757-761.
He X, Zhang Y, Gao H, et al. Simultaneous quantification of picfeltarraenins IA and IB in rat plasma by UPLC-MS/MS: Application to a pharmacokinetic study. J Pharm Biomed Anal. 2016;120:32-37.
Fang H, Liang X, Ning D, Chen H. Analysis of picfeltarraenins IA and IB in Picria fel-terrae. Guihaia. 2010;28(5):708-710.
Shi TS. Content determination of picfeltarraenin IA in Xiaoyanling tablets by HPLC. Chin Pharm J. 2012;15(5):674-675.
Zou JM, Wu MJ, Wang LS. HPLC fingerprint of Picria fel-terrae. Chin Pharm J. 2005;40(9):664-666.
Zou JM, Wang LS, Yan H, Yin WQ, Guo YJ. Determination of picfeltarraenins IA and IB in Picria fel-terrae Lour with TLCS. Chin J Pharm Anal. 2005;25:654-656.
Dresler S, Bogucka-Kocka A, Kováčik J, et al. Separation and determination of coumarins including furanocoumarins using micellar electrokinetic capillary chromatography. Talanta. 2018;187:120-124.
Cao K, Xu Y, Mu X, Zhang Q, Wang R, Lv J. Sensitive determination of pyrrolizidine alkaloids in Tussilago farfara L. by field-amplified, sample-stacking, sweeping micellar electrokinetic chromatography. J Sep Sci. 2016;39(21):4243-4250.
Wang ML, Guo J, Wang ZY, et al. Simultaneous separation and determination of hirsutine and hirsuteine by cyclodextrin-modified micellar electrokinetic capillary chromatography. Phytochem Anal. 2020;31(1):112-118.
Gomes AF, Ganzera M, Schwaiger S, et al. Simultaneous determination of iridoids, phenylpropanoids and flavonoids in Lippia alba extracts by micellar electrokinetic capillary chromatography [J]. Microchem J. 2018;138:494-500.
Nishi H, Fukuyama T, Matsuo M, Terabe S. Chiral separation of optical isomeric drugs using micellar electcrokinetic chromatography and bile salts. J Microcolumn Sep. 1989;1(5):234-241.
Guo H, Wang Z, Xu L, Zhang H, Chang R, Chen A. Separation and simultaneous determination of seven bioactive components in Tripterygium wilfordii Hook. F. and Tripterygium preparations by micellar electrokinetic capillary chromatography. Electrophoresis. 2019;40(4):547-554.
Gomes AF, Ganzera M, Schwaiger S, et al. Simultaneous determination of iridoids, phenylpropanoids and flavonoids in Lippia alba extracts by micellar electrokinetic capillary chromatography. Microchem J. 2018;138:494-500.
De Oliveira A, Silva CA, Silva AM, Tavares MF, Kato MJ. Development and validation of a micellar electrokinetic chromatography method for quantitative determination of butenolides in Piper malacophyllum (C. Presl) C. DC. Phytochem Anal. 2010;21(5):428-433.
Liu Z, Zou H, Ye M, Ni J, Zhang Y. Effects of organic modifiers on solute retention and electrokinetic migrations in micellar electrokinetic capillary chromatography. Electrophoresis. 1999;20(14):2898-2908.
Liu Z, Zou H, Ye M, Ni J, Zhang Y. Effects of organic modifiers on retention mechanism and selectivity in micellar electrokinetic capillary chromatography studied by linear solvation energy relationships. J Chromatogr A. 1999;863(1):69-79.
Hu SQ, Shi HJ, Yu H, Dong J, Guo XM. Solvent-modified MEKC for the enantioseparation of palonosetron hydrochloride and related enantiomeric impurities. Electrophoresis. 2015;36(21-22):2762-2767.
Tonin FG, Jager AV, Micke GA, Farah JP, Tavares MF. Optimization of the separation of flavonoids using solvent-modified micellar electrokinetic chromatography. Electrophoresis. 2005;26(17):3387-3396.
Zhao J, Yang G, Liu H, Wang D, Song X, Chen Y. Determination of andrographolide, deoxyandrographolide and neoandrographolide in the Chinese herb Andrographis paniculata by micellar electrokinetic capillary chromatography. Phytochem Anal. 2002;13(4):222-227.