Freezing on the beach: A robust coral sperm cryopreservation design.
3D printing
Conservation
Coral sperm
Cryobanking
Cryopreservation
Cryoprotectant
Cryovial
Freezing rate
Journal
Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
received:
10
04
2020
revised:
17
03
2021
accepted:
15
04
2021
pubmed:
23
4
2021
medline:
23
9
2021
entrez:
22
4
2021
Statut:
ppublish
Résumé
Cryopreservation of coral sperm requires reliable, travel-ready, inexpensive hardware. To this end, we developed and tested a robust, second-generation, conduction-based cryovial cooling rack assembled from 3D-printed and commercially available parts. Cooling rates from -10 to -80 °C were found to be repeatable at -22.9 ± 1.9 (rate ± SD) °C/min for 1-mL samples and -35.4 ± 3.3 °C/min for 0.5-mL samples. This represents an improvement on the variability of cooling rates in an older design, which was found to be -31.8 ± 7.1 °C/min for 1-mL samples. Design files and a manual were produced to encourage widespread use and the development of derivative designs.
Identifiants
pubmed: 33887237
pii: S0011-2240(21)00077-8
doi: 10.1016/j.cryobiol.2021.04.005
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
135-139Informations de copyright
Copyright © 2021 Smithsonian Institution. Published by Elsevier Inc. All rights reserved.