Routine laboratory test enabling the detection of dermatophytes and the identification of Trichophyton rubrum by means of in-house duplex real-time PCR.
CFX96®
Dermatophytes
Duplex real-time PCR
Pre-treatment PCR
Trichophyton rubrum
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
01
01
2021
revised:
15
04
2021
accepted:
16
04
2021
pubmed:
23
4
2021
medline:
15
12
2021
entrez:
22
4
2021
Statut:
ppublish
Résumé
Dermatophytes are responsible, in majority, for fungal infections of skin, hair and nails, and Trichophyton rubrum is the most frequently isolated dermatophyte in humans. The time for dermatophyte growth in culture requires a total of two to four weeks. Molecular methods were developed to improve time to diagnosis and initiation of treatment. We present here an in-house duplex real-time PCR enabling detection of dermatophytes and simultaneous identification of T. rubrum from mycological samples and cultures. The objective of this work was to optimize the fungal DNA extraction method, the detection of dermatophytes and the identification of T. rubrum on a CFX96® (Real-Time PCR Detection System). In addition, the method comparison showed that this new method is more sensitive than the culture and microscopic observations. To conclude, this routinely used method has been accredited ISO 15189 since January 2020 in our laboratory.
Identifiants
pubmed: 33887312
pii: S0167-7012(21)00097-X
doi: 10.1016/j.mimet.2021.106229
pii:
doi:
Substances chimiques
DNA, Fungal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106229Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.