Confocal microscopy 3D imaging of diesel particulate matter.


Journal

Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769

Informations de publication

Date de publication:
Jun 2021
Historique:
received: 22 07 2020
accepted: 16 04 2021
pubmed: 24 4 2021
medline: 29 6 2021
entrez: 23 4 2021
Statut: ppublish

Résumé

To date, diesel particulate matter (DPM) has been described as aggregates of spherule particles with a smooth appearing surface. We have used a new colour confocal microscope imaging method to study the 3D shape of diesel particulate matter (DPM); we observed that the particles can have sharp jagged appearing edges and consistent with these findings, 2D light microscopy demonstrated that DPM adheres to human lung epithelial cells. Importantly, the slide preparation and confocal microscopy method applied avoids possible alteration to the particles' surfaces and enables colour 3D visualisation of the particles. From twenty-one PM

Identifiants

pubmed: 33890224
doi: 10.1007/s11356-021-14025-y
pii: 10.1007/s11356-021-14025-y
pmc: PMC8222012
doi:

Substances chimiques

Air Pollutants 0
Particulate Matter 0
Vehicle Emissions 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

30384-30389

Subventions

Organisme : Barts Charity, UK
ID : MGU0312
Organisme : The Medical College of Saint Bartholomew's Hospital Trust
ID : 17/LO/1752

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Auteurs

Lisa Miyashita (L)

Centre for Genomics and Child Health, Blizard Institute, Queen Mary University of London, London, UK.

Gary Foley (G)

Centre for Genomics and Child Health, Blizard Institute, Queen Mary University of London, London, UK.

Ian Gill (I)

Faculty of Science, Engineering and Computing, Kingston University, Surrey, KT1 2EE, UK.

Gavin Gillmore (G)

Faculty of Science, Engineering and Computing, Kingston University, Surrey, KT1 2EE, UK.
School of Science, Bath Spa University, Bath, UK.

Jonathan Grigg (J)

Centre for Genomics and Child Health, Blizard Institute, Queen Mary University of London, London, UK.

David Wertheim (D)

Faculty of Science, Engineering and Computing, Kingston University, Surrey, KT1 2EE, UK. D.Wertheim@kingston.ac.uk.

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