Transcription factor OTX2 silences the expression of cleavage embryo genes and transposable elements.
OTX2
Totipotency
Zygotic genome activation
Journal
The Journal of reproduction and development
ISSN: 1348-4400
Titre abrégé: J Reprod Dev
Pays: Japan
ID NLM: 9438792
Informations de publication
Date de publication:
21 Jun 2021
21 Jun 2021
Historique:
pubmed:
27
4
2021
medline:
15
12
2021
entrez:
26
4
2021
Statut:
ppublish
Résumé
Upon mammalian fertilization, zygotic genome activation (ZGA) and activation of transposable elements (TEs) occur in early embryos to establish totipotency and support embryogenesis. However, the molecular mechanisms controlling the expression of these genes in mammals remain poorly understood. The 2-cell-like population of mouse embryonic stem cells (mESCs) mimics cleavage-stage embryos with transient Dux activation. In this study, we demonstrated that deficiency of the transcription factor OTX2 stimulates the expression of ZGA genes in mESCs. Further analysis revealed that OTX2 is incorporated at the Dux locus with corepressors for transcriptional inhibition. We also found that OTX2 associates with TEs and silences the subtypes of TEs. Therefore, OTX2 protein plays an important role in ZGA and TE expression in mESCs to orchestrate the transcriptional network.
Identifiants
pubmed: 33896883
doi: 10.1262/jrd.2021-007
pmc: PMC8238675
doi:
Substances chimiques
DNA Transposable Elements
0
Otx Transcription Factors
0
Otx2 protein, mouse
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
223-228Références
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