Proteomic Profiling of IgG1 Producing CHO Cells Using LC/LC-SPS-MS
CHO cells
SPS-MS3
TMT
bioprocess monitoring
monoclonal antibody
protein characterization
proteomic profiling
Journal
Frontiers in bioengineering and biotechnology
ISSN: 2296-4185
Titre abrégé: Front Bioeng Biotechnol
Pays: Switzerland
ID NLM: 101632513
Informations de publication
Date de publication:
2021
2021
Historique:
received:
02
06
2020
accepted:
19
03
2021
entrez:
26
4
2021
pubmed:
27
4
2021
medline:
27
4
2021
Statut:
epublish
Résumé
The biopharmaceutical market is dominated by monoclonal antibodies, the majority of which are produced in Chinese hamster ovary (CHO) cell lines. Intense cell engineering, in combination with optimization of various process parameters results in increasing product titers. To enable further improvements in manufacturing processes, detailed information about how certain parameters affect cellular mechanisms in the production cells, and thereby also the expressed drug substance, is required. Therefore, in this study the effects of commonly applied changes in bioprocessing parameters on an anti-IL8 IgG1 producing CHO DP-12 cell line were investigated on the level of host cell proteome expression combined with product quality assessment of the expressed IgG1 monoclonal antibody. Applying shifts in temperature, pH and dissolved oxygen concentration, respectively, resulted in altered productivity and product quality. Furthermore, analysis of the cells using two-dimensional liquid chromatography-mass spectrometry employing tandem mass tag based isotopic quantitation and synchronous precursor selection-MS
Identifiants
pubmed: 33898396
doi: 10.3389/fbioe.2021.569045
pmc: PMC8062983
doi:
Types de publication
Journal Article
Langues
eng
Pagination
569045Informations de copyright
Copyright © 2021 Strasser, Farrell, Ho, Scheffler, Cook, Pankert, Mowlds, Viner, Karger and Bones.
Déclaration de conflit d'intérêts
JH, KS, KC, RV, PP, and PM are employed by Thermo Fisher Scientific, the company that develops and produces the MAbPac SCX, Acclaim and Easy Spray Acclaim columns, Vanquish UHPLC system and Orbitrap mass spectrometers. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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