Proteomic Changes Associated With Sperm Fertilizing Ability in Meat-Type Roosters.
chicken
fertility
proteomic
semen
sperm
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2021
2021
Historique:
received:
19
01
2021
accepted:
03
03
2021
entrez:
26
4
2021
pubmed:
27
4
2021
medline:
27
4
2021
Statut:
epublish
Résumé
The molecular basis of male fertility remains unclear, especially in chickens, where decades of genetic selection increased male fertility variability as a side effect. As transcription and translation are highly limited in sperm, proteins are key molecules defining their functionality, making proteomic approaches one of the most adequate methods to investigate sperm capacity. In this context, it is interesting to combine complementary proteomic approaches to maximize the identification of proteins related to sperm-fertilizing ability. In the present study, we aimed at identifying proteins related to fertility in meat-type roosters, showing fertility variability. Fertile roosters (fertility rates higher than 70% after artificial insemination) differed from subfertile roosters (fertility rates lower than 40%) in their sperm mass motility. Fertile and subfertile sperm protein contents were compared using two complementary label-free quantitative proteomic methods: Intact Cell MALDI-TOF-Mass Spectrometry and GeLC-MS/MS. Combining the two strategies, 57 proteins were identified as differentially abundant. Most of them were described for the first time as differentially abundant according to fertility in this species. These proteins were involved in various molecular pathways including flagellum integrity and movement, mitochondrial functions, sperm maturation, and storage in female tract as well as oocyte-sperm interaction. Collectively, our data improved our understanding of chicken sperm biology by revealing new actors involved in the complexity of male fertility that depends on multiple cell functions to reach optimal rates. This explains the inability of reductionist
Identifiants
pubmed: 33898456
doi: 10.3389/fcell.2021.655866
pmc: PMC8063615
doi:
Types de publication
Journal Article
Langues
eng
Pagination
655866Informations de copyright
Copyright © 2021 Vitorino Carvalho, Soler, Thélie, Grasseau, Cordeiro, Tomas, Teixeira-Gomes, Labas and Blesblois.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Mol Cell Proteomics. 2016 Jun;15(6):1998-2010
pubmed: 27044871
Theriogenology. 2008 Jan 15;69(2):252-61
pubmed: 17977587
J Proteomics. 2018 Mar 20;175:56-74
pubmed: 28385661
Mol Reprod Dev. 2009 Apr;76(4):334-41
pubmed: 18932200
J Proteomics. 2012 Oct 22;75(18):5861-71
pubmed: 22796355
Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20497-502
pubmed: 23169663
Data Brief. 2016 Aug 16;8:1421-5
pubmed: 27617276
Biol Reprod. 2020 Apr 15;102(4):852-862
pubmed: 31837139
J Proteomics. 2017 Jun 6;162:125-134
pubmed: 27576136
FASEB J. 2007 Apr;21(4):1013-25
pubmed: 17244819
Poult Sci. 1985 Jan;64(1):155-8
pubmed: 3975194
Reproduction. 2002 Jan;123(1):79-86
pubmed: 11869189
J Assist Reprod Genet. 2014 May;31(5):549-54
pubmed: 24658925
J Proteomics. 2015 Jan 1;112:313-35
pubmed: 25086240
J Proteome Res. 2013 Nov 1;12(11):4738-47
pubmed: 24016215
Nat Biotechnol. 2014 Mar;32(3):223-6
pubmed: 24727771
Connect Tissue Res. 2001;42(4):255-67
pubmed: 11913770
Biol Reprod. 2007 Dec;77(6):906-13
pubmed: 17715429
Anal Chem. 2002 Oct 15;74(20):5383-92
pubmed: 12403597
Biochim Biophys Acta Gen Subj. 2017 Feb;1861(2):147-156
pubmed: 27836756
Curr Biol. 2008 Jun 24;18(12):911-4
pubmed: 18571413
Mol Reprod Dev. 2008 Apr;75(4):632-40
pubmed: 17924400
J Cell Biol. 2013 Apr 15;201(2):263-78
pubmed: 23569216
Reprod Biomed Online. 2018 Mar;36(3):327-339
pubmed: 29336995
Reproduction. 2008 Oct;136(4):391-9
pubmed: 18614626
J Proteome Res. 2016 Nov 4;15(11):3961-3970
pubmed: 27490519
Cell Motil Cytoskeleton. 2005 Sep;62(1):48-64
pubmed: 16080206
J Chromatogr A. 2014 Oct 3;1362:194-205
pubmed: 25201255
Asian J Androl. 2010 Jan;12(1):59-63
pubmed: 20111082
Theriogenology. 2019 Oct 1;137:30-35
pubmed: 31285051
Biol Reprod. 2006 Aug;75(2):270-8
pubmed: 16687649
Reprod Domest Anim. 2018 Jun;53(3):617-623
pubmed: 29431233
Andrology. 2015 Nov;3(6):1068-75
pubmed: 26445132
PLoS One. 2012;7(11):e50851
pubmed: 23209833
PLoS One. 2014 Mar 07;9(3):e91302
pubmed: 24608277
J Proteomics. 2016 Apr 14;138:124-35
pubmed: 26926441
Anim Reprod Sci. 2015 Oct;161:75-81
pubmed: 26364125
Bioinformatics. 2012 Sep 1;28(17):2270-1
pubmed: 22796955
Mol Reprod Dev. 2009 May;76(5):453-9
pubmed: 18951373
Mol Reprod Dev. 1997 Aug;47(4):490-6
pubmed: 9211434
Anal Chem. 2004 Jul 15;76(14):4193-201
pubmed: 15253663
Fertil Steril. 2003 Sep;80(3):531-5
pubmed: 12969693
J Reprod Immunol. 2016 Nov;118:100-108
pubmed: 27821286
Mol Cell Proteomics. 2014 Sep;13(9):2513-26
pubmed: 24942700
Mol Reprod Dev. 2019 Aug;86(8):999-1012
pubmed: 31134708
Anal Chem. 2003 Sep 1;75(17):4646-58
pubmed: 14632076
Cell Stress Chaperones. 2011 Mar;16(2):173-9
pubmed: 20890741
BMC Genomics. 2014 Oct 14;15:897
pubmed: 25315394
Theriogenology. 2020 Jul 1;150:113-121
pubmed: 32284210
Int J Mol Sci. 2020 Sep 24;21(19):
pubmed: 32987677
Fertil Steril. 2013 Mar 15;99(4):998-1007
pubmed: 23415969
Theriogenology. 2019 Nov;139:106-112
pubmed: 31401475
Acta Biochim Biophys Sin (Shanghai). 2011 May;43(5):346-53
pubmed: 21444326
Mol Cell Proteomics. 2020 Jun;19(6):1035-1046
pubmed: 32312844
Dev Biol. 2005 Aug 1;284(1):126-42
pubmed: 15982649
Andrology. 2019 Jul;7(4):454-462
pubmed: 30924599