Employing nanobodies for immune landscape profiling by PET imaging in mice.

Animals Histological Techniques Immunologic Techniques / methods Mice Molecular Imaging / methods Organ Specificity Positron-Emission Tomography / methods Single-Domain Antibodies / analysis
Antibody Immunology Model Organisms Molecular Biology Molecular/Chemical Probes

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
18 06 2021
Historique:
entrez: 26 4 2021
pubmed: 27 4 2021
medline: 27 4 2021
Statut: epublish

Résumé

Noninvasive immunoimaging holds great potential for studying and stratifying disease as well as therapeutic efficacy. Radiolabeled single-domain antibody fragments (i.e., nanobodies) are appealing probes for immune landscape profiling, as they display high stability, rapid targeting, and excellent specificity, while allowing extremely sensitive nuclear readouts. Here, we present a protocol for radiolabeling an anti-CD11b nanobody and studying its uptake in mice by a combination of positron emission tomography imaging,

Identifiants

DOI: 10.1016/j.xpro.2021.100434 PMID: 33899016 PMC: PMC8056265
pubmed: 33899016
doi: 10.1016/j.xpro.2021.100434
pii: S2666-1667(21)00141-6
pmc: PMC8056265
doi:

Substances chimiques

Single-Domain Antibodies 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100434

Subventions

Organisme : NCI NIH HHS
ID : K22 CA226040
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA220234
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL144072
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL131478
Pays : United States

Informations de copyright

© 2021 The Authors.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

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Auteurs

Abraham J P Teunissen (AJP)

Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Omar B Abousaway (OB)

Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215-5418, USA.

Jazz Munitz (J)

Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Mandy M T van Leent (MMT)

Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Yohana C Toner (YC)

Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Bram Priem (B)

Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Department of Medical Biochemistry, Amsterdam UMC, Amsterdam 1105 AZ, the Netherlands.
Angiogenesis Laboratory, Amsterdam UMC, Cancer Center Amsterdam, Amsterdam 1105 AZ, the Netherlands.

Max L Senders (ML)

Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Carlos Pérez-Medina (C)

Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid 28029, Spain.

Willem J M Mulder (WJM)

Biomedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Department of Pharmacological Sciences and Mount Sinai Center for Therapeutics Discovery, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Mohammad Rashidian (M)

Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215-5418, USA.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Employing nanobodies for immune landscape...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Employing nanobodies for immune landscape...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Employing nanobodies for immune landscape...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Employing nanobodies for immune landscape...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Imagerie moléculaire › Employing nanobodies for immune landscape...
questionsmedicales.fr › Phénomènes physiologiques › Spécificité d'organe › Employing nanobodies for immune landscape...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Techniques de diagnostic radioisotopique › Scintigraphie › Tomoscintigraphie › Tomographie par émission de positons › Employing nanobodies for immune landscape...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Fragments d'immunoglobuline › Fragments Fab d'immunoglobuline › Anticorps à domaine unique › Employing nanobodies for immune landscape...