Toll-like receptor 4-mediated cytokine synthesis and post-stroke depressive symptoms.


Journal

Translational psychiatry
ISSN: 2158-3188
Titre abrégé: Transl Psychiatry
Pays: United States
ID NLM: 101562664

Informations de publication

Date de publication:
26 04 2021
Historique:
received: 29 12 2020
accepted: 09 04 2021
revised: 27 03 2021
entrez: 27 4 2021
pubmed: 28 4 2021
medline: 29 6 2021
Statut: epublish

Résumé

Altered cytokine synthesis thought to contribute to the pathophysiology of post-stroke depression (PSD). Toll-like receptor 4 (TLR4) is a master regulator of innate immunity. The aim of this study was to explore the putative association between TLR4-mediated cytokine synthesis and subsequent symptoms of PSD. In total, 262 patients with ischemic stroke and without a history of PSD were included. Depressive symptoms were assessed using the Patient Health Questionnaire-9 in 170 patients on Day 8 and in 146 at 3 months after stroke. Blood samples taken on Day 3 after stroke were stimulated ex vivo with lipopolysaccharide (LPS). Ex vivo synthesized cytokines (TNFα, IP-10, IL-1β, IL-6, IL-8, IL-10, and IL-12p70) and circulating cytokines (TNFα, IL-6, sIL-6R, and IL-1ra) were measured using the enzyme-linked immunoassay or cytometric method. RNA sequencing was used to determine the gene expression profile of LPS-induced cytokines and chemokines. LPS-induced cytokine synthesis and the gene expression of TLR4-dependent cytokines and chemokines did not differ between patients with and without greater depressive symptoms. The plasma level of IL-6, but not TNFα, sIL-6R, and IL-1ra, was higher in patients who developed depressive symptoms at 3 months after stroke (median: 4.7 vs 3.4 pg/mL, P = 0.06). Plasma IL-6 predicted the severity of depressive symptoms at 3 months after stroke (β = 0.42, P = 0.03). In conclusion, TLR4-dependent cytokine synthesis was not associated with greater post-stroke depressive symptoms in this study. Circulating IL-6 might be associated with depressive symptoms occurring at 3 months after stroke.

Identifiants

pubmed: 33903586
doi: 10.1038/s41398-021-01359-x
pii: 10.1038/s41398-021-01359-x
pmc: PMC8076201
doi:

Substances chimiques

Cytokines 0
Lipopolysaccharides 0
TLR4 protein, human 0
Toll-Like Receptor 4 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

246

Subventions

Organisme : Narodowe Centrum Nauki (National Science Centre)
ID : 2015/19/B/NZ4/00287

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Auteurs

Michal Korostynski (M)

Department of Molecular Neuropharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland.

Dzesika Hoinkis (D)

Intelliseq sp. z o.o, Krakow, Poland.

Marcin Piechota (M)

Department of Molecular Neuropharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland.

Slawomir Golda (S)

Department of Molecular Neuropharmacology, Maj Institute of Pharmacology, Polish Academy of Sciences, Krakow, Poland.

Joanna Pera (J)

Department of Neurology, Jagiellonian University Medical College, Krakow, Poland.

Agnieszka Slowik (A)

Department of Neurology, Jagiellonian University Medical College, Krakow, Poland.

Tomasz Dziedzic (T)

Department of Neurology, Jagiellonian University Medical College, Krakow, Poland. dziedzic@cm-uj.krakow.pl.

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Classifications MeSH